利科增强了猪体细胞核转移胚胎中的表观遗传重编程和胚胎基因组激活
Ji Hyeon Yun1,2, Hyo-Gu Kang1,3, Eun Young Choi1,4
1Futuristic Animal Resource & Research Center (FARRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Cheongju, 28116, Republic of Korea.
Scientific reports
|September 26, 2025
概括
烯补充剂通过增强表观遗传重编程和胚胎基因组激活 (ZGA) 来改善猪胚胎的发育. 这种抗氧化剂治疗促进了囊胚细胞的形成,并减少了体细胞核转移 (SCNT) 胚胎中的亡.
科学领域:
- 生殖生物学 生殖生物学
- 发展生物学 发展生物学
- 动物科学动物科学
背景情况:
- 体细胞核转移 (SCNT) 对于产生疾病模型至关重要,但其效率受到表观遗传重编程和胚胎基因组激活 (ZGA) 较差的阻碍.
- 猪胚胎需要最佳的体外培养 (IVC) 条件来克服发育障碍.
- 像莱科这样的抗氧化剂可以减轻氧化应激,改善胚胎发育.
研究的目的:
- 调查烯在IVC期间对猪胚胎发育的影响.
- 为了确定烯是否可以增强SCNT和帕氏基因活性 (PA) 胚胎中的表观遗传重编程和ZGA.
- 阐明莱科对胚胎发育作用的基本机制.
主要方法:
- 在实验室中培养了巴氏基因活化 (PA) 和SCNT猪胚胎,度不同,度不同.
- 评估了胚胎发育参数 (裂变,胚芽细胞形成,细胞数量,细胞亡).
- 分析了活性氧物种 (ROS),线粒体潜力,自,基因表达 (抗氧化剂,表观遗传,ZGA相关) 和DNA甲基化.
主要成果:
- 0.2微米的甘显著改善了裂变速率,芽细胞形成和细胞数量,同时降低了细胞亡.
- 百合素降低了ROS水平,增强了线粒体膜潜力,并促进了自.
- 百合素调节了表观遗传标记 (H3K4me3,H3K9me3,5mC) 并改变了关键重编程和ZGA相关基因的表达.
结论:
- 在IVC期间的烯治疗显著提高了猪SCNT和PA胚胎的发育.
- 百合素通过减少氧化应激,改善线粒体功能,诱导自,促进核重编程和ZGA来改善胚胎发育.
- 百合素作为一种补充剂,有望提高猪中基于SCNT的胚胎生产效率.
相关概念视频
Methods of Nuclear Reprogramming
2.1K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.1K
Somatic to iPS Cell Reprogramming
2.6K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.6K
Introduction to Nuclear Reprogramming
2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K
Chromatin Modification in iPS Cells
2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K


