相关实验视频
Updated: Jan 16, 2026

07:44
Identifying Protein-protein Interaction Sites Using Peptide Arrays
Published on: November 18, 2014
18.5K
与VDAC1相互作用的蛋白质:结合部位映射及其衍生会诱导细胞亡和多方面的细胞效应
Manikandan Santhanam1, Venkatadri Babu1, Anna Shteinfer-Kuzmine2
1Department of Life Sciences, Ben-Gurion University of the Negev, 84105, Beer-Sheva, Israel.
Apoptosis : an international journal on programmed cell death
|September 27, 2025
概括
研究人员确定了VDAC1相互作用蛋白的特定结合部位. 从这些部位获得的可以调节VDAC1活动,影响细胞死亡和潜在治疗应用的信号通路.
科学领域:
- 线粒体生物学 线粒体生物学
- 蜂信号传输是如何进行的
- 蛋白质与蛋白质的相互作用
背景情况:
- 线粒体电压依赖性离子通道-1 (VDAC1) 对于调节细胞新陈代谢,能量产生和亡至关重要.
- VDAC1与细胞区内的众多蛋白质相互作用,整合了线粒体和细胞功能.
- 了解这些相互作用是解读细胞调节和开发向治疗的关键.
研究的目的:
- 在互动的合作伙伴上确定特定的VDAC1结合点.
- 通过细胞透来研究向这些VDAC1结合部位的功能后果.
- 探索调节VDAC1-蛋白相互作用的治疗潜力.
主要方法:
- 设计了一个由19个VDAC1相互作用蛋白质的768个基组成的基阵列.
- 专注于从GAPDH,gelsolin和actin中获得的.
- 评估了与纯化VDAC1的相互作用及其对细胞死亡,Ca2+和ROS水平,转录因子和蛋白质表达等细胞过程的影响.
主要成果:
- VDAC1结合诱导细胞死亡,增加细胞内Ca2+和ROS,并增强p53和c-Jun转录因子.
- 可以促进VDAC1的上调和寡合化,从而触发细胞亡.
- 来自GAPDH的并不影响催化活性; 来自凝的改变了actin组织; 来自actin的影响了细胞骨蛋白表达.
结论:
- 这项研究是第一个在19种相互作用蛋白质上绘制VDAC1结合位点的研究.
- 衍生作为细胞透工具来调节VDAC1网络.
- 准VDAC1-蛋白相互作用是一个新的治疗策略.
相关概念视频
The Intrinsic Apoptotic Pathway
8.3K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
8.3K
The Extrinsic Apoptotic Pathway
8.1K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
8.1K
Caspases
13.7K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
13.7K
Apoptosis
14.1K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
14.1K
Autophagic Cell Death
4.3K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
4.3K
Phagocytosis of Apoptotic Cells
4.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
4.9K

