在酵母中定义一种简化的过程,用于生产包裹的VLP登革热疫苗
Salomé de Sá Magalhães1, Stephen A Morris1, Shinta Kusumawardani2,3
1Department of Biochemical Engineering, Faculty of Engineering Sciences, University College London, London WC1E 6BT, UK.
Bioengineering (Basel, Switzerland)
|September 27, 2025
概括
这项研究引入了Komagataella phaffii (Pichia pastoris) 作为登革热疫苗生产的新型宿主. 这种酵母平台为全球卫生需求提供了高效,低成本的登革热病毒样颗粒 (VLP) 制造.
科学领域:
- 生物技术是生物技术.
- 疫苗开发 疫苗开发
- 微生物发酵 微生物发酵
背景情况:
- 登革热是一个日益严重的全球健康问题,每年有数百万病例报告.
- 目前的登革热疫苗制造在效率,成本和可扩展性方面面临挑战.
- 需要创新的生产方法来满足全球需求.
研究的目的:
- 探索为登革热疫苗制造的替代工艺开发途径.
- 用 Komagataella phaffii (Pichia pastoris) 作为宿主生物来生产登革热疫苗.
- 为应对可扩展疫苗制造的技术和运营挑战.
主要方法:
- 证明了Komagataella phaffii在产生登革热病毒样颗粒 (VLP) 的多功能性.
- 在小型生物反应堆中使用实验设计 (DOE) 优化培养过程.
- 开发了一种用于封装VLP (eVLP) 的新型净化平台,并建立了生物物理特征方法.
主要成果:
- 使用Komagataella phaffii平台成功产生登革热病毒样颗粒 (VLPs).
- 优化发酵条件,提高VLP产量和质量.
- 建立了封装VLP (eVLP) 的强有力的净化策略.
结论:
- 科马加泰拉法菲为登革热疫苗制造提供了一个有前途,可扩展和具有成本效益的平台.
- 开发的方法为应对当前疫苗生产挑战提供了基础.
- 这项研究为有效的全球登革热疫苗供应铺平了道路.
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