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以TMT为基础的蛋白质组学分析对Cryphonectria parasitica的囊泡蛋白质表达模式进行低病毒感染的评估

Zishan Zeng¹, Nanxin Lin¹, Tao Lu²

¹Guangxi Key Laboratory for Polysaccharide Materials and Modifications, School of Marine Sciences and Biotechnology, Guangxi Minzu University, Nanning 530008, China.

|September 27, 2025

概括

低病毒感染改变了栗子菌 (Cryphonectria parasitica) 的囊泡运输系统. 这影响了关键的细胞过程,可能解释了 reduced真菌毒性.

科学领域:

菌类学菌类学是指菌类学.
病毒学病毒学
分子生物学分子生物学

背景情况:

低病毒感染降低了 *Cryphonectria parasitica * 的致病性,这是导致栗子病的真菌.
之前的研究表明,宿主线粒体和囊泡中的病毒蛋白p48异型有助于病毒性衰减.

研究的目的:

为了研究与低病毒感染相关的真菌囊泡中的蛋白质变化.
了解病毒感染如何影响C. parasitica*中的囊泡运输系统和细胞功能.

主要方法:

基于Tandem Mass Tag (TMT) 的定量蛋白质学被用来识别和量化囊泡中的真菌蛋白质.
总共有1739种真菌蛋白被确定.

主要成果:

低病毒CHV1-EP713感染导致C. parasitica*囊泡中的75个上调和201个下调的蛋白质.
受影响的蛋白质主要涉及囊泡运输,蛋白质折叠,膜融合,自和ER压力.
具体的蛋白质变化包括对calnexin,COPI,ArfGAP,importin-β和Atg8的下调,以及对clathrin,dynamin,Vps10p,HSP70和t-SNAREs的上调.

结论:

低病毒感染会导致C. parasitica的囊泡运输系统发生显著的改变.
这些改变是由关键蛋白调节器的变化介导的,可能会影响至关重要的细胞过程,并导致观察到的毒性减弱.

关键词:

这种寄生虫是Cryphonectria parasitica.不同的蛋白质组学分析.病毒下降的病毒.囊泡介导的细胞内传输.

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