牧场诱导的circRNAs,miRNAs和mRNAs表达的变化 在西藏绵羊双腿骨中表达
Xiong Ma1, Shaobin Li2, Zhanzhao Chen2
1Faculty of Chemistry and Life Sciences, Gansu Minzu Normal University, Hezuo 747000, China.
Biology
|September 27, 2025
概括
放牧显著增加了西藏羊肉中的肌肉内脂肪 (IMF),可能是通过促进I型肌肉纤维. 特定的圆形RNAs (circRNAs) 和microRNAs (miRNAs) 被确定为在放牧条件下肉质的关键调节者.
科学领域:
- 动物科学动物科学
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 肉质是畜牧业生产的一个关键特征.
- 了解影响肉品质的分子机制,例如肌内脂肪 (IMF) 含量,对于优化生产至关重要.
- 西藏羊是一种有价值的品种,通过改善放牧等养策略来提高它们的肉质感兴趣很大.
研究的目的:
- 为了研究放牧与养对西藏绵羊肉质量的影响.
- 确定与在放牧环境下改善肉质相关的分子机制,特别是RNA表达特征和调控网络.
- 探索圆形RNAs (circRNAs) 和microRNAs (miRNAs) 在调节西藏羊的肌肉纤维特征和能量代谢中的作用.
主要方法:
- 在放牧和养条件下对西藏绵羊进行比较分析.
- 使用索克斯莱特提取方法量化肌内脂肪 (IMF) 含量.
- 转录组测序以分析RNA表达特征.
- 构建一个竞争的内源性RNA (ceRNA) 调控网络.
主要成果:
- 与养相比,放牧显著增加了西藏绵羊双腿肌肌中的IMF含量.
- 在放牧组中,一些circRNAs (例如, novel_circ_001331, novel_circ_012918) 被上调调.
- 鉴定的circRNAs和miRNAs可能通过MAPK,Wnt和VEGF信号通路调节参与肌肉纤维类型切换和能量代谢的基因 (例如,COL8A1,MYLK3,NOX4).
结论:
- 放牧积极影响西藏绵羊肉质量,可能是通过促进I型肌肉纤维的形成.
- 特定的circRNA和miRNA在放牧条件下对肉质的调节作用.
- 这项研究为了解肉质监管中的circRNA和miRNA功能提供了基础,并为改善西藏绵羊生产提供了见解.
相关概念视频
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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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