Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Effect of Aging on Tissues01:19

The Effect of Aging on Tissues

Several body functions deteriorate with age. The external signs of aging are easily identifiable. For example, the skin becomes dry, less elastic, and thins out, forming wrinkles. The skin of the face begins to appear looser due to a decrease in the levels of elastic and collagen fibers in the connective tissue. Additionally, melanin production in the hair follicle decreases with age, resulting in gray hair. Moreover, the senses of sight and hearing decline, so glasses and hearing aids may...
Bone Disorders01:29

Bone Disorders

Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
Bone deposition is also affected by the levels of sex hormones like estrogen and testosterone that promote osteoblast activity and bone matrix synthesis. When the level of these hormones decreases due to aging, it causes a reduction in bone deposition. As a result, bone resorption by osteoclasts...
Menopause01:28

Menopause

Menopause, a natural biological process marking the end of a woman's fertility, typically occurs between the fifth and sixth decade of life. This phase is characterized by the exhaustion of the ovarian follicle pool, leading to less responsive ovaries despite the high levels of Follicle Stimulating Hormone (FSH) and Luteinizing Hormone (LH). The consequential decrease in estrogen production results in symptoms like hot flashes, heavy sweating, headaches, hair loss, muscle pains, vaginal...
Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Distribution01:00

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Distribution

Drug distribution in the human body is influenced by several factors, including plasma protein concentration, body composition, blood flow, tissue-protein concentration, and tissue fluid pH. Among these, changes in plasma protein concentration and body composition due to aging significantly affect how drugs are distributed within the body. Specifically, aging is associated with a decrease in albumin levels by about 10% and an increase in α1-acid glycoprotein levels. These alterations are not...
Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Excretion01:18

Pharmacokinetics in Geriatric Patients: Effect of Age on Drug Excretion

In geriatric patients, renal physiology undergoes significant changes, including diminished renal blood flow and a lower glomerular filtration rate (GFR), leading to alterations in medication clearance. Drugs such as aminoglycoside antibiotics, lithium, and digoxin, which rely on glomerular filtration for removal from the body, particularly impact pharmacokinetics. These drugs tend to have slower clearance rates in older adults, necessitating careful dosage considerations.Evaluation of renal...
Pharmacodynamics in Geriatric Patients: Effects of Age01:27

Pharmacodynamics in Geriatric Patients: Effects of Age

Age-related pharmacokinetic changes are extensively documented, but understanding age-related pharmacodynamic alterations is relatively limited. This knowledge gap can be partly attributed to the complexity of developing appropriate measures of drug responses compared to bioanalytical methods for determining drug concentrations.Most information regarding age-related differences in human pharmacodynamics originates from cross-sectional studies. However, these studies assume that observed mean...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Early Post-Transplant Peripheral B-Cell Profiles in Kidney Transplant Recipients: Clinical Associations and Limitations.

Journal of clinical medicine·2026
Same author

Unsupervised Immune Profiling Identifies Distinct Post-Transplant T-Cell Clusters Associated with Kidney Allograft Function.

Medical sciences (Basel, Switzerland)·2026
Same author

Small Bowel Lesions and Bleeding Risk in Hemodialysis Patients: A Narrative Review.

Hemodialysis international. International Symposium on Home Hemodialysis·2026
Same author

Diagnostic Performance of Interleukin-6 (IL-6) and Membrane Glycoprotein Cluster of Differentiation-64 (CD64) for Acute Appendicitis in Girls Presenting with Lower Abdominal Pain.

Diagnostics (Basel, Switzerland)·2026
Same author

Diagnostic and Prognostic Value of Donor-Derived Cell-Free DNA in Acute Rejection After Kidney Transplantation: A Narrative Review.

Diagnostics (Basel, Switzerland)·2026
Same author

Cellular Immunity in Chronic Kidney Disease and Changes After Kidney Transplantation.

Transplant international : official journal of the European Society for Organ Transplantation·2026

相关实验视频

Updated: Jun 30, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

34.9K

测量与衰老相关的分泌表现型

Achilleas Karras1,2, Georgios Lioulios3, Konstantia Kantartzi1,4

  • 1School of Medicine, Democritus University of Thrace, 68100 Alexandroupolis, Greece.

Biomedicines
|September 27, 2025
PubMed
概括

细胞衰老,一个关键的衰老因素,涉及一个复杂的分泌表型 (SASP). 本综述详细介绍了测量SASP组件的方法,这些方法对于了解衰老和开发老年疗法至关重要.

关键词:
免疫性 免疫性 免疫性免疫染剂的免疫染剂.蛋白质分析分析 蛋白质分析定量逆转录的转录方式与衰老相关的分泌表现型

更多相关视频

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.5K
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

4.0K

相关实验视频

Last Updated: Jun 30, 2026

Techniques to Induce and Quantify Cellular Senescence
06:51

Techniques to Induce and Quantify Cellular Senescence

Published on: May 1, 2017

34.9K
A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence
13:59

A Quantitative Measurement of Reactive Oxygen Species and Senescence-associated Secretory Phenotype in Normal Human Fibroblasts During Oncogene-induced Senescence

Published on: August 12, 2018

8.5K
Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry
08:52

Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

Published on: April 6, 2022

4.0K

科学领域:

  • 老年学和细胞生物学,专注于衰老机制和细胞反应.

背景情况:

  • 细胞衰老是衰老的标志,通过衰老相关的分泌表型 (SASP) 驱动组织功能障碍.
  • SASP是一种复杂的分泌分子混合物,根据细胞类型和环境而异,构成测量挑战.
  • 了解SASP对于老龄化研究和开发老年治疗策略至关重要.

研究的目的:

  • 为测量SASP组件的方法提供全面的概述.
  • 讨论不同生物水平和样本类型的各种检测技术的优点和局限性.
  • 确定当前SASP检测策略中的差距,并建议未来的研究方向.

主要方法:

  • 在转录 (qRT-PCR,RNA测序,in situ杂交),转化 (ELISA,西方抹杀,质谱,Luminex,MSD) 和功能层面测量SASP的技术审查.
  • 分析适用于细胞培养,组织和系统液体的方法.
  • 包括空间检测方法,如免疫组织化学和免疫光学.

主要成果:

  • SASP测量涉及分子水平 (RNA,蛋白质) 和样本来源 (细胞,组织,流体) 的各种方法.
  • 每种方法都有特定的优势和局限性,影响SASP量化准确性和范围.
  • 多参数方法对于全面了解衰老细胞活动至关重要.

结论:

  • 准确的SASP量化非常重要,但由于其复杂性和上下文依赖性,具有挑战性.
  • 当前的方法提供了各种视角,但没有一种方法能够捕捉到SASP的全部范围.
  • 为了推进衰老和疾病研究,特别是生物标志物发现,需要进一步完善SASP检测策略.