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Updated: Jan 16, 2026

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Published on: September 14, 2013

从大型动物兽医教学医院环境中分离出Clostridioides difficile

Alexandre S Borges¹, Luiza S Zakia¹, Serena Yu¹

¹Departments of Clinical Studies, Ontario Veterinary College, University of Guelph, Guelph, ON N1G 2W1, Canada.

Animals : an open access journal from MDPI

|September 27, 2025

概括

在大型动物医院的8.3%的表面中发现了困难菌 (C. difficile). 这凸显了加强生物安全措施的必要性,以预防兽医环境中的医院感染.

科学领域:

兽医医学兽医医学兽医医学
传染性疾病传染性疾病
环境微生物学环境微生物学

背景情况:

困难菌 (C. difficile) 构成兽医医院内病房感染的重大风险,但其环境流行程度尚不清楚.
有限的研究已经调查了C. difficile在兽医医院环境中的存在和特征.

研究的目的:

在安大略省兽医学院的大型动物医院环境中确定C. difficile的流行率.
鉴定复原的C. difficile分离物,包括毒素基因存在,核型,序列类型和抗菌素耐药性.

主要方法:

一个大型动物诊所内的157个表面地点的环境采样.
使用选择性介质和多重PCR对毒素基因 (tcdA,tcdB,cdtA/cdtB) 的培养.
分子特征包括PCR-Ribotyping,多位置序列类型 (MLST) 和抗菌素耐药性预测.

主要成果:

在采样地点的8.3% (13/157) 检测到C. difficile.
确定了十种不同的核糖类型,其中核糖类型014和010是最常见的.
七个分离物中含有毒素基因A和B;所有这些都对二进制毒素基因负面. 隔离物属于MLST Clade 1,发现了5种不同的序列类型. 在一些隔离品中,发现了抗菌素耐药性基因的高患病率.

结论:

困难菌在大型动物医院的各个区域存在,特别是高流量区域和难以清洁的表面.
积极的监测和强大的生物安全协议对于最大限度地减少环境污染至关重要.
实施这些措施对于预防兽医机构的医院C. difficile感染至关重要.

关键词:

厌氧性厌氧性厌氧性马类的马类动物.临床医院医疗机构.子子是一种子.

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