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在液态保存的野猪精液中使用细胞学污点对精子形态的比较评估

Annika Braune¹, Axel Wehrend¹, Johannes Kauffold²

¹Veterinary Clinic for Reproductive Medicine and Neonatology, Justus-Liebig-University of Giessen, 35392 Giessen, Germany.

Animals : an open access journal from MDPI

|September 27, 2025

概括

青是用于野猪精液形态的最实用和最经济的染料. 虽然其他污点有好处,但Eosin为例行评估提供了成本,速度和有效性的最佳平衡.

科学领域:

兽医科学兽医科学兽医科学
生殖生物学生殖生物学
动物科学动物科学

背景情况:

精子形态的准确评估对于猪的人工授精至关重要.
猪精液的液体保存需要可靠的染色技术进行评估.

研究的目的:

为了比较猪精液形态学的九种常见染色方法.
根据精度,成本,时间和存储稳定性来评估污点.

主要方法:

在324个野猪精液样本上测试了9种染色技术.
立即进行形态评估,并在储存1天,1周,3个月后进行形态评估.

主要成果:

乙是最快,最具成本效益的,具有良好的对比度,但可以更好地检测变化.
氨酸-黑素提供了详细的形态,但显示了随着时间的推移而形成的晶体.
血色色素失去了色素清晰度,Sangodiff-G的对比度很差,Spermac耗时,Formol-Citrate-Rose Bengal是不稳定的,Testsimplets的解释性下降,Methyl Violet缺乏分辨率和稳定性.

结论:

乙是常规猪精液形态评估的最实用和最经济的选择.
氨酸-尼格洛辛是有效的,但由于储存不稳定性而受到限制.
污点选择应与实验室的特定需求和约束相一致.

关键词:

野猪野猪野猪野猪野猪新鲜的新鲜的新鲜的新鲜光显微镜光学显微镜精子精子精子染色方法染色方法储存储存储存储存是一个

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