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Updated: Jan 16, 2026

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

A Bioinformatics Pipeline for Investigating Molecular Evolution and Gene Expression using RNA-seq

Published on: May 28, 2021

嗅觉罗塞特的比较转录学揭示了草食和肉食鱼类的表达分歧和适应性进化Xenocyprididae鱼类

Hua Xue¹, Hailong Gu², Liu Yang¹

¹Shanghai Universities Key Laboratory of Marine Animal Taxonomy and Evolution, Shanghai Ocean University, Shanghai 201306, China.

Animals : an open access journal from MDPI

|September 27, 2025

概括

这项研究揭示了鱼类的嗅觉基因如何根据饮食而不同地演变. 它强调了嗅觉系统在适应中的作用,并提供了关于鱼类养行为的见解.

科学领域:

*比较基因组学和转录基因组学
* 进化生物学进化生物学
* 鱼类生物学鱼类生物学

背景情况:

* 嗅觉对于鱼类的养和适应至关重要.
*关于食草鱼与食肉鱼的嗅觉基因调节和进化研究有限.
*Xenocyprididae家族为饮食差异化研究提供了一个模型.

研究的目的:

* 为了研究Xenocyprididae鱼的嗅觉系统的转录调节和分子演变.
* 为了比较食草和食肉物种之间的嗅觉基因表达和进化速率.
* 了解嗅觉系统在饮食适应中的作用.

主要方法:

*四种Xenocyprididae物种 (两种食草动物,两种食肉动物) 的嗅觉罗塞特转录组测序.
*对单基因,正基因和嗅觉相关基因进行跨物种比较分析.
*分析基因表达趋势 (RNA-Seq) 和进化速率 (Ka/Ks).
* 定量逆转录PCR (qRT-PCR) 用于验证.

主要成果:

* 确定了每种8-19个嗅觉受体基因,富含嗅觉转导通路.
*观察到跨物种基因表达差异,不严格按饮食分类.
*积极选择的基因参与免疫反应和神经调节;嗅觉基因 *oard1* 显示积极选择.
*不同表达的基因与RNA处理,代谢物运输和外来生物代谢有关.

结论:

* 嗅觉系统在基本的嗅觉之外的各种适应性反应中发挥着作用.
*基因表达和进化速率为嗅觉系统适应提供了互补的见解.
*这项研究为了解淡水鱼类饮食适应的感知基础提供了基础.

关键词:

在RNA-seqqq.它们属于Xenocyprididae.养适应养适应嗅觉系统的嗅觉系统阳性选择是一种积极的选择.一个副本的正本正本.

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