通过用Eurotium cristatum进行浸泡发酵,对三种不同类型的发酵茶进行比较分析
Jiahong Lan1, Mingwei Xie1, Xudong Li1
1College of Tea and Food Science, Wuyi University, 358# Baihua Road, Wuyishan 354300, China.
Foods (Basel, Switzerland)
|September 27, 2025
概括
由Eurotium cristatum发酵的轻度发酵茶 (LFT) 显示出增强的抗氧化能力和独特的代谢特征. 由于其丰富的生物活性代谢物,LFT最适合功能性抗氧化剂饮料.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物化学 生物化学
背景情况:
- 发酵茶由于微生物的转变,提供了多种健康益处.
- 克里斯塔 (Eurotium cristatum) 是传统发酵食品和饮料中的一个关键微生物.
- 了解E. cristatum对不同茶叶发酵水平的代谢影响对于开发功能性产品至关重要.
研究的目的:
- 通过使用Eurotium cristatum,研究轻度,半度和高度发酵茶的代谢概况和抗氧化特性.
- 为了确定关键的生物活性代谢物,负责增强抗氧化活性.
- 为了确定生产功能性抗氧化剂饮料的最佳发酵水平.
主要方法:
- 三种茶类 (LFT,SFT,HFT) 的浸泡发酵 (SmF) 使用纯E.cristatum.
- 用于化学分析的非目标代谢学.
- 抗氧化剂测定 (例如DPPH,ABTS).
- 网络药理学和分子对接分析.
- 感官评估. 感官评估.
主要成果:
- 与对照组相比,所有发酵茶输液都显示出显著增强的抗氧化能力.
- 对于LFT,SFT和HFT观察到不同的代谢概况,LFT显示了最多的上调代谢物.
- 17种生物活性代谢物,包括Phloretin和Epicatechin gallate,与抗氧化活性有很强的相关性.
- 网络药理学确定了15种潜在的有益健康的抗氧化剂代谢物,主要是在LFT中.
- 根据代谢物概况和感官评估,LFT表现出对功能性抗氧化剂饮料生产的最高适应性.
结论:
- 欧克里斯塔的发酵显著改变了化学成分,并增强了茶叶灌注的抗氧化能力.
- 最初的化学成分会影响发酵,但E. cristatum的微生物调解在塑造最终产品方面占主导地位.
- 轻度发酵茶 (LFT) 是开发功能性抗氧化剂饮料的最有前途的基质,因为它具有卓越的代谢物概况和抗氧化活性.
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