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Updated: Jan 16, 2026

Identification of Hemolytic and Phospholipase Activity in Crude Extracts from Sea Anemones by Straightforward Bioassays
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贝的活性:隔离,净化,识别和细胞保护功能在受H2O2损坏的细胞上

Chan Bai1, Wenqing Wang1,2, Guowei Huang1,3

  • 1Key Laboratory of Cold Chain Logistics Technology for Agro-Product, Ministry of Agriculture and Rural Affairs, Institute of Agro-Product Processing and Nuclear Agricultural Technology, Hubei Academy of Agricultural Sciences, No.5 Nanhu Avenue, Hongshan District, Wuhan 430064, China.

Biomolecules
|September 27, 2025
PubMed
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贝贝酸显示出强大的抗氧化剂和ACE抑制作用. 这些生物活性可以作为促进健康的抗高血压和抗氧化剂的天然成分.

科学领域:

  • 生物化学 生物化学
  • 食品科学 食品科学 食品科学
  • 药理学 药理学是指药理学的学科.

背景情况:

  • 贝是一种丰富的蛋白质来源,具有潜在的生物活性特性.
  • 从海鲜副产品中开发功能成分解决了废物利用和健康促进问题.

研究的目的:

  • 开发和描述具有增强抗氧化剂和血管素-I转化酶 (ACE) 抑制活性的贝.
  • 研究这些的潜力作为抗高血压和抗氧化剂.

主要方法:

  • 编制和按分子量分成的甲蛋白水解剂 (CSPH).
  • 使用DPPH和ABTS激素清除试验来评估抗氧化活性.
  • 测量了ACE抑制活性,并使用LC-MS/MS识别了活性.
  • 细胞测试 (HUVECs, HepG2) 用于评估氧化 (NO),内甲素-1 (ET-1) 和活性氧物种 (ROS) 的抑制,以及对氧化损伤的保护.

主要成果:

  • CSPH2 (3-5 kDa) 显示出显著的抗氧化剂和 ACE 抑制活性.
  • 鉴定了纯化的抗氧化剂 (APAPLPPPAP) 和 ACE 抑制剂 (QGPDDPLIPIM) .
  • 这些增加了NO,降低了ET-1,抑制了HUVEC中的ROS,并保护了HepG2细胞免受氧化应激.
关键词:
生物活动是生物活动.贝贝类类的化物.细胞保护作用 细胞保护作用质谱测量识别识别 质谱测量识别

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结论:

  • 贝贝酸具有强大的抗氧化剂和ACE抑制特性.
  • 这些具有作为抗高血压和抗氧化剂应用中的功能成分的潜力.
  • 这项研究强调了鱼加工副产品的健康益处和利用.