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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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相关实验视频

Updated: Jan 16, 2026

Correlative Optical Spectroscopy and Mass Spectrometry Imaging Methodology to Visualise Drug Distribution in a Soft Tissue Section
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使用总离子计数图像的TOF-SIMS深度分析图像的深度校正.

Melanie A Brunet1, Brittney L Gorman2, Mary L Kraft1,2

  • 1Department of Chemical and Biomolecular Engineering, University of Illinois Urbana-Champaign, Champaign, IL 61801, USA.

Biomolecules
|September 27, 2025
PubMed
概括
此摘要是机器生成的。

本研究介绍了一种方法来纠正细胞的二次离子质谱 (TOF-SIMS) 图像的3D深度分析时间的z轴扭曲. 精确的3D TOF-SIMS对ER-PM结节等细胞结构的成像现在是可能的.

关键词:
在TOF-SIMS深度分析中.深度纠正 纠正深度 纠正深度细胞内网膜-血膜结合点 细胞内网膜-血膜结合点

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相关实验视频

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科学领域:

  • 细胞生物学 细胞生物学
  • 分析化学 分析化学
  • 生物物理学的生物物理.

背景情况:

  • 飞行二次离子质谱的深度分析时间 (TOF-SIMS) 是一种用于成像细胞内的代谢物分布的强大技术.
  • 来自TOF-SIMS数据的3D重建经常遭受z轴扭曲,阻碍了对细胞结构的准确解释.

研究的目的:

  • 开发和验证一种方法来纠正z轴扭曲在3D TOF-SIMS深度分析图像的细胞.
  • 提高3D重建的准确性,以可视化亚细胞结构,特别是内网膜-血膜 (ER-PM) 结合点.

主要方法:

  • 利用TOF-SIMS深度分析的总离子图像来构建一个3D细胞表面形态模型.
  • 应用了形态模型来纠正特定组件的3D TOF-SIMS图像中的z位置和voxels高度.
  • 在一个简化的蜂模型中测试了ER-PM连接的3D TOF-SIMS数据上的校正方法.

主要成果:

  • 开发的方法成功地纠正了3D TOF-SIMS图像中的z轴扭曲.
  • 与未经纠正的图像相比,深度校正的3D图像提供了更准确的ER-PM连接结构表示.
  • 将更正的图像投射到形态模型上,可视化了相对于细胞表面特征的结点.

结论:

  • 精确的,特定于组件的3D图像现在可以从深度分析TOF-SIMS数据集中生成.
  • 这种校正方法有助于在ER-PM连接处识别脂质和代谢物.
  • 这些发现可能有助于更好地理解代谢物在神经元功能中的作用.