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无标签和超灵敏的APE1检测基于混合化连锁反应与G-四重复合相结合

Yarong Zhang¹, Hongyan Ma¹, Zhenyao Gao²

¹Key Laboratory of Analytical Technology and Detection of Yan'an, College of Chemistry and Chemical Engineering, Yan'an University, Yan'an 716000, China.

|September 27, 2025

概括

研究人员开发了一种新型生物传感器,用于超敏感检测阿普里尼克/阿普里米尼克内核酶1 (APE1) 的新型生物传感器. 这种无酶,无标签的方法在生物样本中实现了APE1检测的高灵敏度.

科学领域:

生物化学生物化学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

阿普里尼克/阿普里米尼克内核酶1 (APE1) 对于DNA修复和保持基因组稳定性至关重要.
APE1的失调与各种疾病有关,包括癌症和心血管疾病.
目前的APE1检测方法缺乏某些应用所需的灵敏度.

研究的目的:

开发一个创新的生物传感平台,用于超敏感检测APE1.1.
为了实现APE1.1.的无酶和无标签生物测试.
为APE1抑制剂查和评估提供一个工具.

主要方法:

集成APE1特定的位置识别与杂交连锁反应 (HCR) 信号放大.
一个含有AP位点的发针 (HP) 探头的设计,该探头被APE1.1切割.
在APE1介导的切割时释放HCR启动器以触发信号放大.

主要成果:

实现了APE1检测的超高灵敏度,检测极限为1.0 × 10-8 U/mL.
在复杂的生物基质如细胞溶解物中表现出强大的生物传感器性能.
验证了该平台在查和评估APE1抑制剂方面的适用性.

结论:

开发的生物传感平台为APE1检测提供了高度敏感和高效的方法.
这个平台促进了无酶和无标签的生物测试,克服了现有技术的局限性.
生物传感器是疾病研究和针对APE1.1的治疗开发的宝贵工具.

关键词:

在G-quadruplex中使用.阿普里尼克/阿普里米尼克内核酶1 (APE1)光是一种光.杂交连锁反应 (HCR) 是一种

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