代谢适应决定了多种真核生物中TOR信号的进化轨迹
Kyle Johnson1, Dellaraam Pourkeramati1, Ian Korf1,2
1Department of Molecular and Cellular Biology, College of Biological Sciences, University of California, Davis, CA 95616, USA.
Biomolecules
|September 27, 2025
概括
拉帕米辛复合体2 (TORC2) 的标对于真核生物的异质新陈代谢至关重要. 它通常在严格的自营养菌中丢失,但在混合营养菌和异质营养菌中保留,揭示了对营养素获取的进化压力.
科学领域:
- 单核细胞生物学 单核细胞生物学
- 进化生物学是进化的生物学.
- 生物化学 生物化学
背景情况:
- 细胞的营养获取策略 (自,异,混合) 影响细胞调节网络的进化.
- 拉帕素 (TOR) 激酶的目标,一种保守的生长调节剂,在两个复合体中起作用:TORC1和TORC2.
- 在很大程度上,TORC1是保守的,而TORC2的进化分布不那么明确.
研究的目的:
- 调查TORC1和TORC2在不同真核细胞系中的进化分布和保留模式.
- 为了将TORC2的存在或不存在与不同的营养获取策略和代谢生活方式相关联.
主要方法:
- 开发了一个基于隐藏的马尔科夫模型 (HMM) 的管道,用于敏感检测核心TORC1和TORC2组件.
- 调查了来自主要超群的800多个序列化真核基因组.
- 分析了与代谢策略相关的TOR复合物的存在/缺失 (自性,混合性,异性).
主要成果:
- TORC1和TORC2都存在于早期分支的真核生物中,这表明它们存在于最后一个真核生物共同祖先中.
- 观察到TORC2的多个谱系特异性损失,以及较少的TORC1.
- 在初级光合作用自营菌中,TORC2均缺失,在二级/三级自营菌中经常丢失,但在混合型和异质型自营菌中始终保留.
结论:
- TORC2很可能支持异构代谢,在严格的自身养中是不可用的.
- 在真核生物进化过程中,代谢和生态压力显著影响了TOR复合物的保留.
- 这些发现为了解TOR信号通路的进化分歧提供了一个框架.
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