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便代谢学用于诊断困难菌感染

Carlos Bea-Serrano^1,2, Andreu Belmonte-Domingo¹, Carolina Pinto-Pla¹

¹Internal Medicine Department, Clinic University Hospital of Valencia, INCLIVA Biomedical Research Institute, 46010 València, Spain.

Diagnostics (Basel, Switzerland)

|September 27, 2025

概括

便代谢学可以在住院患者中区分Clostridioides difficile殖民和感染. 这种方法显示了改善C. difficile感染 (CDI) 诊断的希望,使用特定的代谢物签名.

科学领域:

微生物学微生物学
代谢学代谢学代谢学
临床诊断临床诊断临床诊断

背景情况:

艰难闭膜炎感染 (CDI) 是医院获得的腹的主要原因.
目前的诊断很难区分CDI和殖民.
准确的区分对于有效的患者管理至关重要.

研究的目的:

评估便代谢学在住院患者中诊断CDI的有用性.
确定特定的代谢生物标志物,区分CDI与殖民.
评估这些生物标志物的诊断性能.

主要方法:

对患有急性腹的住院成年人进行前性观察研究.
在控制中分层,C. difficile殖民和C. difficile感染的群体.
使用UPLC-MS/MS和GC-MS对代谢物 (SCFA,氨基酸,胆酸) 的便样本分析.
多变量 (PLS-DA,sPLSDA) 和单变量统计分析与FDR校正.

主要成果:

感染患者的SCFA度显著增加,胆酸概况发生变化.
确定了关键的歧视性代谢物:异酸盐,酸盐,异酸盐和α-氨基黄油酸.
对于这些标记物,ROC曲线分析显示出强大的诊断性能 (AUC > 0.85).

结论:

便代谢分析有效地区分了CDI与住院患者的殖民化.
代谢签名有可能提高CDI的诊断精度.
这种方法可以导致更有针对性和及时的治疗干预.

关键词:

克洛斯特里迪奥伊德斯困难菌在SCFAs中,SCFAs是最重要的.氨基酸是氨基酸中的一种.胆汁酸胆汁酸胆汁酸生物标志物生物标志物诊断诊断诊断诊断诊断诊断便代谢物质的代谢物质

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