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综合代谢学和转录学揭示了代谢途径的变化 在氧化应激下,常见的肌肌肉的变化
Yongxiang Liu1, Bing Li1,2, Yiran Hou1,2
1Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Antioxidants (Basel, Switzerland)
|September 27, 2025
概括
过氧化暴露会通过影响有机酸和脂质通路而改变鱼的肌肉代谢,而不是氨基酸或脂肪酸. 这项研究揭示了氧化压力对鱼肌肉的影响,有助于抗氧化剂战略的发展.
科学领域:
- 环境毒理学环境毒理学
- 水生动物生理学 水生动物生理学
- 代谢学和转录学.
背景情况:
- 过氧化 (H2O2) 是水生环境中常见的一种反应性氧物种.
- 2O2可以在水生动物中引起毒理作用,但它对肌肉质量和新陈代谢的影响尚不清楚.
研究的目的:
- 为了研究H2O2诱导的鱼肌氧化应激的分子机制.
- 描述暴露于H2O2.2.的鱼的代谢和基因表达变化.
主要方法:
- 进行了综合代谢和转录分析,对鱼 (Cyprinus carpio) 进行了14天的H2O2暴露.
- 分析脂肪酸和氨基酸成分,确定不同代谢物和基因,并进行路径丰富分析 (GO,KEGG).
主要成果:
- 暴露于H2O2并没有显著改变氨基酸或脂肪酸概况.
- 代谢分析显示了83个上调和89个下调的代谢物,主要是有机酸和脂质,与histidine, purin和glyoxylate代谢有关.
- 转录组分析确定了470个上调调节的和451个下调调节的基因,这些基因富含肌肉发育和氧化酸化,脂肪基因素和PPAR信号通路.
结论:
- 暴露于H2O2会诱导鱼肌肉中的代谢失调,由改变的代谢途径和基因表达体现出来.
- 这项研究提供了关于H2O2毒性的见解,鱼类对氧化应激的适应性反应,以及抗氧化策略的基础.
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