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对Chilo suppressalis (步行者) (Lepidoptera: Pyralidae) 的转录组分析揭示了在寄生虫压力下耐寒机制
Chuan-Lei Dong1,2, Elyar Abil1, Rong Ji1,2
1International Research Center for the Collaborative Containment of Cross-Border Pests in Central Asia, Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Sciences, Xinjiang Normal University, Urumqi 830054, China.
Insects
|September 27, 2025
概括
像Cotesia chilonis这样的寄生虫通过改变基因表达来改变宿主Chilo suppressalis的寒冷耐受性. 关键发现揭示了对内细胞网膜活动和热冲击蛋白的影响.
科学领域:
- 昆虫分子生物学 昆虫分子生物学
- 主体-寄生虫相互作用
- 生理生态生态学生理生态学
背景情况:
- 寄生虫为了生存而操纵宿主生理.
- 科特西亚奇洛尼斯寄生于奇洛 suppressalis,影响其寒冷耐受性.
研究的目的:
- 研究C. suppressalis.中C. chilonis诱导的冷耐性调节的分子机制.
- 确定参与宿主对寄生虫压力反应的基因和途径.
主要方法:
- 寄生虫和非寄生虫C. suppressalis幼虫的转录组测序.
- 不同基因表达分析 (DEGs).
- 基因丰富分析 (GO,KEGG).
- 通过RT-qPCR验证.
- 功能性测试对内质网膜活动和信号传递.
主要成果:
- 主体超冷却点在寄生后变化.
- 确定了507个DEG,包括热冲击蛋白 (HSP) 和信号基因.
- 寄生病压力抑制了内细胞网膜活动.
- HSP表达没有显著改变ER活性或血淋巴Ca2+.
结论:
- 通过分子变化,C. chilonis的寄生性显著影响C. suppressalis的寒冷耐受性.
- 主体反应涉及核糖体生物发生,ER蛋白加工和氧化酸化的变化.
- HSP和信号通路都涉及,但它们在ER/血淋巴调节中的直接功能作用需要进一步研究.
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