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PAFAH1B3存在于线性染色体和异染色体圆形DNA中,并通过EMT促进HCC进展

Dandan Li¹, Huishan Sun², Yingjie Wang³

¹Department of Laboratory Medicine, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College (CAMS & PUMC), Beijing 100730, China.

International journal of molecular sciences

|September 27, 2025

概括

在癌症中越来越多地发现像eccDNA PAFAH1B3这样的外染色体圆形DNA (eccDNA). 这项研究表明,eccDNA PAFAH1B3通过增强PAFAH1B3的表达和调节上皮层-介质细胞转变 (EMT) 来促进肝细胞癌 (HCC) 的进展.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

染色体外圆形DNA (eccDNA) 与各种癌症有关.
肝细胞癌 (HCC) 中eccDNAs的特定作用仍然未得到充分研究.
PAFAH1B3 是一种在癌症生物学中感兴趣的基因.

研究的目的:

为了研究HCC中eccDNAPAFAH1B3的丰度和功能.
确定eccDNA PAFAH1B3对HCC细胞增殖,迁移,入侵和亡的影响.
阐明eccDNA PAFAH1B3影响HCC发育的机制,包括其与上皮-介质细胞转换 (EMT) 的关系.

主要方法:

循环测序用于分析HCC组织中的eccDNA丰度.
基于细胞的测试 (EdU,Transwell,细胞亡) 来评估功能影响.
通过RT-PCR和Western Blot (WB) 来测量基因和蛋白质表达.
异种移植瘤模型和免疫组织化学 (IHC) 用于体内验证.

主要成果:

在HCC中,eccDNA PAFAH1B3的丰度显著增加.
过度表达eccDNAPAFAH1B3促进了HCC细胞的增殖,迁移和入侵,同时抑制了细胞亡.
PAFAH1B3的mRNA水平被上调,PAFAH1B3的抑制抑制了瘤的生长.
PAFAH1B3调制影响了EMT标记物,通过敲击增加了E-cadherin和减少了N-cadherin.

结论:

在促进HCC发生和进展方面,eccDNA PAFAH1B3发挥着重要作用.
该机制涉及PAFAH1B3的增强表达和EMT的调节.
eccDNA PAFAH1B3 代表了HCC的潜在治疗标.

关键词:

在PAFAH1B3B3中,PAFAH1B3B3是PAFAH1B3的第一个类型,PAFAH1B3是PAFAH的第一个类型.在ccDNAsDNAs中.表皮细胞到介质细胞的过渡.肝细胞癌是肝细胞癌.

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