在人类前列腺收缩中STIM/口腔输入系统过活化在良性前列腺激增中前列腺收缩的相关性
José M La Fuente1, Mariam El Assar2,3,4, Argentina Fernández5
1Serviço de Urologia, Centro Hospitalar e Universitário de Santo António (CHUdSA), 4099-001 Porto, Portugal.
抑制STIM/Orai系统减少了良性前列腺增生症 (BPH) 患者的前列腺过度收缩. 这一发现表明了管理与BPH相关的下尿路症状的新治疗标.
科学领域:
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
- 细胞生物学 细胞生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 良性前列腺增生 (BPH) 导致前列腺扩大和下泌尿道症状 (LUTS).
- 前列腺超收缩性是与BPH相关的LUTS的潜在贡献者.
- 结构相互作用分子 (STIM) /口腔入系统在BPH相关收缩中的作用尚未完全理解.
研究的目的:
- 研究抑制STIM/Orai系统对BPH患者前列腺和膀部收缩的影响.
- 将这些效应与没有BPH的器官捐献者的组织进行比较.
- 评估BPH前列腺组织中的STIM-1,Orai1和Orai3蛋白表达.
主要方法:
- 从BPH患者和器官捐赠者 (ODs) 获得的人类前列腺 (HP) 和膀部 (HB) 条带的同度张力记录.
- 使用YM-58483 (20μM) 来抑制STIM/Orai系统.
- 西方涂抹和免疫光测定STIM-1,Orai1和Orai3蛋白质的表达和定位.
主要成果:
- STIM/Orai 抑制显著降低了 BPH 患者的 HP 中因诺拉上腺素引起的和神经性收缩.
- 这些抑制作用并未在OD患者的HP或BPH患者的HB中观察到.
- 与对照人群相比,BPH患者的HP显著增加STIM-1蛋白质表达.
结论:
- 在BPH存在时,STIM/Orai系统在前列腺收缩调中发挥着重要的功能作用,可能是由于STIM-1表达增加.
- 抑制STIM/Orai系统可能通过减少前列腺过度收缩来提供BPH管理的治疗策略.
- 准STIM/Orai通路为缓解BPH患者的LUTS提供了一个潜在的途径.
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