一种敏感的SPE-LC-MS/MS方法,用于确定人类尿液中选择的兽医药物和其他有机污染物:开发,验证和应用研究
Wojciech Rodzaj1, Małgorzata Wacławik1, Joanna Jurewicz2
1Department of Toxicology, Faculty of Pharmacy, Medical University of Gdańsk, 107 Hallera Street, 80-416 Gdańsk, Poland.
International journal of molecular sciences
|September 27, 2025
概括
一种新的方法量化了人类尿液中的16种环境污染物,用于生物监测. 这种分析有助于评估人类对杀虫剂和阻燃剂等化学品的暴露.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 毒理学 毒理学 毒理学
背景情况:
- 人类暴露于各种环境化学物质需要人类生物监测 (HBM) 的强有力的分析方法.
- 关键污染物包括农药 (如菲普罗尼尔),双和阻燃剂,需要同时量化.
研究的目的:
- 开发和验证液体染色学-并联质谱法 (LC-MS/MS) 方法,用于量化人体尿液中的有机污染物及其代谢物.
- 评估菲普罗尼尔- (FIP-OH) 作为HBM中菲普罗尼尔 (FIP) 暴露的新生物标志物的适用性.
主要方法:
- 酶去结合,然后进行固相提取 (SPE) 用于样品制备.
- 液体染色学-并联质谱学 (LC-MS/MS) 用于同时定量16个目标分析物.
- 方法验证根据准确性,精度和灵敏度的既定指南进行验证.
主要成果:
- 成功开发并验证了人类尿液中16种有机污染物和/或代谢物的LC-MS/MS方法.
- 在目标分析物中达到从0.5到2000 pg/mL的低量化极限 (LOQ).
- 在研究的物主人中,有71%的人检测到菲普尼尔 (Fipronil,FIP) (中位数:3.32 pg/mL);其他化学物质也经常被检测到. 由于潜在的不稳定性,Fipronil-hydroxy (FIP-OH) 不能被包括在内.
结论:
- 经过验证的LC-MS/MS方法适用于人类生物监测研究,以量化尿液中的一系列有机污染物.
- 该方法为评估人类对环境污染物的暴露提供了有价值的数据.
- 需要进一步研究FIP-OH作为生物标志物的稳定性和实用性.
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