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扩展PRF:热量对牙纤维细胞基因表达的影响

Xiaoyu Huang^1,2, Layla Panahipour¹, Dorna Rassi Faghihi¹

¹Department of Oral Biology, University Clinic of Dentistry, Medical University of Vienna, 1090 Vienna, Austria.

International journal of molecular sciences

|September 27, 2025

概括

加热贫血小板血 (PPP) 显著降低其生物活性,正如牙纤维细胞的基因表达变化所示. 这一发现支持使用热处理PPP在扩展的富血小板纤维素 (e-PRF) 中用于指导组织再生.

科学领域:

生物材料科学生物材料科学
细胞生物学细胞生物学
再生医学是一种再生医学.

背景情况:

扩展的富血小板纤维素 (e-PRF) 使用热凝结的贫血小板血 (PPP) 混合与的外套层 (BC).
在e-PRF中经过热处理的PPP的生物活性尚未完全理解.
测试加热PPP降低其生物活性的假设是必要的.

研究的目的:

调查加热对PPP生物活性的影响.
为了比较细胞对常规PPP,加热PPP (hPPP) 和BC的反应.
为了验证e-PRF的临床概念.

主要方法:

将牙纤维细胞暴露在PPP,hPPP和BC的溶解物中.
进行大量RNA测序以分析基因表达变化.
使用RT-PCR和免疫试验确认基因表达变化.

主要成果:

在纤维细胞中,PPP溶解物诱导了显著的基因表达变化 (153个上升,71个下降).
hPPP溶解酸导致基因表达变化最小 (5上升,5下降),表明生物活性被废除.
BC比PPP更强,PPP,但不是hPPP,激活了NF-κB信号传递.

结论:

加热PPP几乎完全取消了它的生物活性.
牙纤维细胞对PPP的生物反应对热量敏感.
这些发现支持e-PRF的临床使用,将生物活性BC与热不活化的PPP结合起来.

关键词:

在TGF-β的基础上.专辑蛋白 (albumin) 是一种这是一种化学物质,chemokine.纤维素纤维素是一种纤维素.血小板缺乏血的血.丰富于血小板的纤维素纤维素.

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