来自乳酸细菌的基于表代谢物的抗菌配方作为对抗耐药性大肠杆菌的多目标战略.
Gabriela N Tenea1, Diana Molina1, Yuleissy Cuamacas1
1Biofood and Nutraceutics Research and Development Group, Faculty of Engineering in Agricultural and Environmental Sciences, Universidad Tecnica del Norte, Av. 17 de Julio s-21, Barrio El Olivo, Ibarra 100150, Ecuador.
Antibiotics (Basel, Switzerland)
|September 27, 2025
概括
来自乳酸细菌的基于外代谢物的配方 (ExAF) 对抗多药耐药细菌表现出强大的抗微生物活性. 这些富含的生物基化合物为对抗耐药性感染提供了有希望的环保替代品.
科学领域:
- 微生物学 微生物学
- 生物技术是生物技术.
- 食品科学 食品科学 食品科学
背景情况:
- 越来越多的抗药性 (MDR) 细菌感染率需要新的抗菌战略.
- 乳酸细菌 (LAB) 产生具有潜在抗菌特性的外代谢物.
- 调查基于外代谢物的配方 (ExAFs) 作为可持续的替代品.
研究的目的:
- 评估来自本地LAB的ExAFs对MDR*Escherichia coli*菌株L1PEag1.1的抗菌疗效.
- 描述ExAFs对细菌细胞产生的结构损伤.
- 使用LC-MS/MS. 在最有效的ExAF中识别生物活性代谢物.
主要方法:
- 选14个ExAFs使用时间杀死测试.
- 通过扫描和传输电子显微镜 (SEM/TEM) 评估细菌超结构损伤.
- 使用LC-MS/MS与SWATH获取对最强效的ExAF (E10) 的代谢分析.
主要成果:
- 来自*Weissella cibaria* UTNGt21O的ExAF E10显示出最强的抑制 (17.12 ± 0.22毫米的抑制区域).
- 对于E1和E10在1×MIC时观察到的快速杀菌活性 (>98%在2-3小时内减少) .
- SEM/TEM揭示了膜破坏和细胞内损伤,表明了膜向机制.
- 对E10的代谢分析确定了22种生物活性化合物,包括和黄,与RiPPs有相似之处.
结论:
- ExAFs,特别是E10,是强大的,富含的,生物基抗菌候选药物.
- RiPP类型的存在表明了多方面的杀菌作用.
- 在食品安全和治疗方面,ExAFs代表了有希望的环保替代品,用于管理MDR病原体.
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