相关实验视频

Updated: Jan 16, 2026

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

来自俄罗斯医院的Vancomycin-ResistantEnterococcus faecium与完整和截断的vanA操作子分离的基因组流行病学

Anna Slavokhotova¹, Andrey Shelenkov¹, Yulia Mikhaylova¹

¹Central Research Institute of Epidemiology, Novogireevskaya Str., 3a, 111123 Moscow, Russia.

Antibiotics (Basel, Switzerland)

|September 27, 2025

概括

在俄罗斯的医院中,抗米素的Enterococcus faecium (VREfm) 显示出高的基因组可塑性和耐药性. 基因组监测对于跟踪VREfm传播和了解抗菌素耐药性的演变至关重要.

科学领域:

微生物学微生物学
基因组学就是基因组学.
流行病学流行病学

背景情况:

抗菌素的Enterococcus faecium (VREfm) 是一个重要的全球医院威胁,特别是凡A阳性菌株.
作为一种病原体,E. faecium的成功与其环境弹性,基因组可塑性和抗菌素耐药性获得有关.
了解俄罗斯的VREfm流行病学非常重要,因为它的流行率越来越高.

研究的目的:

来自莫斯科医院的vanA阳性VREfm临床分离物的全基因组特征.
分析这些分离物的遗传关系,抗微生物耐药性概况,毒性因子和等离子体含量.
为俄罗斯的VREfm流行病监测提供基线基因组数据.

主要方法:

10个临床VREfm分离物的全基因组测序 (WGS).
多部位序列类型 (MLST) 和核心基因组MLST (cgMLST) 用于遗传学分析.
表型和基因组抗微生物耐药性概况,毒性因子分析和等离子体含量确定.

主要成果:

所有的分离物属于CC17系 (ST80和ST552).
两个分离物中含有截断的Tn1546转位子,表明潜在的新兴的vanA操作变体.
长读数测序使得能够重建载有截断的vanA操作子的等离子体.

结论:

基因组调查对于追踪VREfm耐药机制和传播至关重要.
该研究提供了俄罗斯VREfm监测的基本基线数据.
持续的基因组监测是必要的,以了解肠球菌中抗菌素耐药性的演变.

关键词:

MLST的类型编写抗微生物耐药性抗微生物耐药性与医疗保健相关的感染截断的 vanA 操作子.毒性因素是毒性的因素.整个基因组的测序.

更多相关视频

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Author Spotlight: Advancing Antibiotic Resistance Research Using an Efflux-Deficient Bacterial Strain and a Single-Copy Gene Expression System

Published on: January 5, 2024

相关概念视频

Development of Antibiotic Resistance

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...