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从牛乳中分离的Serratia marcescens:抗菌耐药性和毒性特征
Guilherme Moreira1, Luís Pinho1, João R Mesquita1,2,3
1School of Medicine and Biomedical Sciences, ICBAS-UP, University of Porto, Rua de Jorge Viterbo Ferreira, nr. 228, 4050-313 Porto, Portugal.
Antibiotics (Basel, Switzerland)
|September 27, 2025
概括
这项研究确定了牛乳腺炎 (BM) 中的新型抗菌素耐药性 (AMR),导致Serratia marcescens分离物. 基因组分析揭示了关键的AMR和毒性因素,将BM菌株与环境Serratia marcescens联系起来.
科学领域:
- 兽医微生物学 兽医微生物学
- 基因组学就是基因组学.
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 牛乳炎 (BM) 是乳牛群的重要经济疾病.
- 塞拉蒂亚马尔塞森斯是一种新兴的病原体,导致BM.
- 在BM病原体中抗菌素耐药性 (AMR) 的增加是一个越来越令人担忧的问题.
研究的目的:
- 为了研究牛乳炎的AMR和毒性因素,Serratia marcescens分离物.
- 为了进行全基因组测序 (WGS) 和 BM 隔离物的基因组学分析.
- 确定新的AMR模式和BM相关的S. marcescens的潜在来源.
主要方法:
- 使用MicroScan WalkAway Plus对四种格兰阴性分离物的表型特征.
- 针对AMR和病毒性因子 (VF) 基因识别的全基因组测序 (WGS).
- 遗传学分析用于比较孤立的基因组及其起源.
主要成果:
- 确定的S. marcescens和S. odorifera从牛乳腺炎中分离出来.
- 检测到对多种药物的表型抗微生物耐药性,包括托布拉米辛,赛福洛キシ姆,胆素和尼托福兰.
- 通过WGS发现了特定的AMR基因 (例如,AAC(6') -Ic,qxB,blaSST-1) 和VF基因 (例如,flgH,fliP).
- 遗传学分析表明BM S. marcescens分离物与环境菌株密切相关.
结论:
- 这项研究报告了BM S. marcescens中第一个对托布拉米辛,素,素和酸的AMR实例.
- 基因组分析证实了多个AMR和VF基因的存在,强调了分离物的致病潜力.
- 遗传学数据表明,牛乳腺炎S. marcescens和环境S. marcescens菌株之间存在联系.
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