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甲状腺功能过高的小鼠模型中的NES-428乳杆菌的免疫调节作用:对格雷夫斯病的潜在应用

Min-Gyu Lee¹, Dong-Hyun Lee¹, Suzie Kang¹

¹School of Life Sciences and Biotechnology, Korea University, Anam-dong, Sungbuk-gu, Seoul 02841, Republic of Korea.

|September 27, 2025

概括

来自金的新型乳酸细菌NES-428通过调节免疫反应,有效地减少小鼠的甲状腺功能障碍. 这种潜在的益生菌可能为管理格雷夫斯病提供了一种新的方法.

科学领域:

微生物学微生物学
免疫学免疫学免疫学
内分泌学在内分泌学.

背景情况:

自免疫性甲状腺功能障碍缺乏安全的,基于微生物组的治疗方法.
一种新型的乳酸细菌,NES-428 (Lactobacillus brevis),从金中分离出来.
它的免疫调节和抗甲状腺潜力需要评估.

研究的目的:

评估NES-428对T细胞细胞因子产生的免疫调节作用.
在小鼠模型中评估NES-428在改善实验性甲状腺功能障碍的疗效.
在体内调查NES-428对甲状腺功能和免疫细胞反应的影响.

主要方法:

用热杀死NES-428预先调节Jurkat T细胞,并刺激它们评估细胞因子转录水平.
在小鼠中使用编码人类TSH受体的腺病毒 (Ad-TSHR) 诱导实验性甲状腺功能过高症.
小鼠每天接受15周的口服NES-428,并对血清甲状腺素,细胞细胞因子和甲状腺组织病理学进行评估.

主要成果:

在Jurkat细胞中,NES-428预先条件显著下调IL-2和IFN-γ转录,同时增加IL-4.
在甲状腺功能高的小鼠中,NES-428治疗将血清甲状腺素水平降低了约50% (p < 0.001).
NES-428使甲状腺组织病理正常化,恢复体重增加,并将囊细胞细胞因子分泌转向IL-4主导型.

结论:

来自金奇的NES-428菌株表现出显著的免疫调节活性,减弱了Th1扭曲反应.
在小鼠模型中,NES-428有效地改善了实验性甲状腺功能障碍.
这些发现支持进一步调查NES-428作为在Graves病中免疫调节的益生菌候选者.

关键词:

格雷夫斯的疾病.牛乳杆菌 (Lactobacillus brevis) 是一种甲状腺功能过强症甲状腺功能过强症免疫调节免疫调节免疫调节益生菌是一种益生菌.

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