关于"Lentilactobacillus buchneri"KU200793作为潜在益生菌的全面安全评估
Suin Kim1, Huijin Jeong1, Na-Kyoung Lee2
1Department of Food Science and Biotechnology, Gachon University, Seongnam 13120, Republic of Korea.
Microorganisms
|September 27, 2025
概括
益生菌菌株Lentilactobacillus buchneri KU200793显示出良好的安全性,没有检测到有害活动. 然而,其抗四环素耐药性需要进一步调查安全益生菌的使用.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 益生菌 益生菌 益生菌
背景情况:
- Lentilactobacillus buchneri KU200793表现出神经保护作用,需要对潜在的益生菌应用进行彻底的安全评估.
- 全面的安全评估对于确定新型益生菌菌株的适用性至关重要.
研究的目的:
- 通过表型和基因型方法全面评估Lentilactobacillus buchneri KU200793的安全性.
- 评估与该菌株抗生素耐药性特征相关的风险.
主要方法:
- 现型分析包括对血液溶解,粘素降解,醇生产,凝酶活性,尿酶活性和生物氨基酸生产的测试.
- 基因型分析利用HGTree程序来识别抗生素耐药性基因并评估它们的同质性.
- 生物氨基胺分析的重点是精子胺,也评估了胆盐脱.
主要成果:
- 从表型来看,L. buchneri KU200793 没有表现出血溶性,蛋白溶性,尿素酶或醇活性,并且产生了极少的 D-乳酸盐和精氨酸.
- 这种菌株没有解胆盐,支持其安全性.
- 基因型分析确定了tetA(58) 和nalD基因,但具有较低的同质性,表明耐药性不是来自水平基因转移.
结论:
- L. buchneri KU200793对于益生菌的使用具有普遍有利的安全性,缺乏关键的毒性因子.
- 鉴定出的四环素耐药性不太可能是可转移的,从而最大限度地降低了传播风险.
- 建议进行进一步的安全评估,以确认L. buchneri KU200793作为益生菌的适用性.
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