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对大肠杆菌RNA片段的转录后3'-终端修饰为了增强多样性而演化

Nikita M Kamoldinov^1,2, Valery V Panyukov^1,3, Nikolay P Kolzhetsov¹

¹Department of Functional Genomics of Prokaryotes, Institute of Cell Biophysics of the Russian Academy of Sciences in Federal Research Center Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences, 142290 Pushchino, Russia.

|September 27, 2025

概括

研究人员在大肠杆菌中发现了经过修改的氨酸tRNA碎片,具有独特的3'扩展. 这些碎片影响细菌在微生物群中的持久性,这表明它们在原始防御机制中发挥了作用.

科学领域:

微生物生物学微生物生物学
RNA生物学的RNA生物学
细菌遗传学细菌遗传学

背景情况:

新兴的研究重点是从RNA衍生出来的功能性寡核酸.
这些RNA片段可以经历修改,扩大它们的细胞作用.
大肠杆菌中的Dps蛋白影响RNA片段的分泌.

研究的目的:

调查野生型和dps-null Escherichia coli 中的差异性细胞内和细胞外RNA片段.
识别新型RNA片段及其修改.
探索这些RNA片段在微生物群落中的功能影响.

主要方法:

对野生型和dps-null Escherichia coli 的差分RNA分析.
在RNA-seq数据中检测可变RNA终端的计算管道的开发.
在人造微生物群中对合成RNA片段进行ex vivo测试.

主要成果:

发现了具有随机3'-终端延伸的氨酸tRNA片段,依赖Dps分泌.
观察到经过修改的 LeuT ((VPQ) tRNA 片段的细胞内丰富,受生长介质和细菌竞争的影响.
鉴定出其他产生有异质末端的寡头的基因组位点,表明末端延长不仅限于LeuT(VPQ).
合成 LeuT ((VPQ) 的类似物刺激了人工微生物群中的多种类型的持久性,这种效应因3'端延长而减弱.

结论:

对RNA片段的非模板扩展可以通过类似干扰的机制扩大用于消除mRNA的目标分子.
这些扩展可以产生从大肠杆菌基因组中缺少的新型序列,可能作为一个原始的防御系统.
这些发现突出了修饰tRNA片段在细菌相互作用和防御中的新型调节作用.

关键词:

3′末端的扩展.在 DPS 找 DPS.仿制的寡核酸是化学的.氨酸的tRNAs可以被使用.通过多核化.转录后RNA的修改.它们分泌的RNAs.

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