激情果 (Passiflora edulis) 中BAG基因家族的进化和功能动力学
Munsif Ali Shad1,2, Songguo Wu2, Yuxin Wu3
1Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region, Shaoguan 512005, China.
Plants (Basel, Switzerland)
|September 27, 2025
概括
研究人员确定了15个激情果BAG (Bcl-2-关联乙原体) 基因,揭示了它们在植物发育和应激反应,特别是热应激中的作用. 这一发现有助于增强激情果的耐热性.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 生物化学 生物化学
背景情况:
- Bcl-2-关联的乙原蛋白 (BAG) 蛋白质是关键的合作伙伴,参与细胞过程,如生长,发育和亡.
- 虽然人类的BAG基因家族已被广泛研究,但植物的BAG同类基因仍然在很大程度上未被探索,尽管它们在进化过程中得到了保护.
- 激情果是一种具有经济意义的作物,使其遗传资源对农业改进有价值.
研究的目的:
- 在激情果 (Passiflora edulis) 中对BAG基因家族进行全面的全基因组识别和表征.
- 在各种压力条件下调查激情果BAG (PeBAG) 基因的表达模式.
- 探索PeBAG基因在提高激情果对环境压力的耐受性,特别是热量的潜力.
主要方法:
- 全基因组扫描用于识别PeBAG基因.
- 生物信息分析以表征PeBAG基因结构,域 (BAG,ubiquitin,calmodulin-binding) 和家族遗传关系.
- 转录组数据分析和定量实时PCR (qRT-PCR) 以评估在荷尔蒙,非生物和热应激下基因表达.
- 在植物中进行亚细胞定位实验.
主要成果:
- 在激情果中发现了15个PeBAG基因,其中大多数存在于多个副本中.
- 根据域组成,PeBAG成员被分为两组:第1组含有无素域和第2组含有卡尔莫杜林结合域.
- PeBAG基因在茎,花和果实中显示出显著的表达. 在荷尔蒙,非生物和热应激下,PeBAG4-3和PeBAG6-1被上调,由qRT-PCR证实.
- 亚细胞局部化揭示了多样化的细分 (核,细胞质,塑),表明功能分歧.
结论:
- 这项研究首次全面描述了激情果中BAG基因家族的特征.
- 特定的PeBAG基因 (PeBAG4-3和PeBAG6-1) 被热应激激活,这表明它在应激反应中的作用.
- 已识别的PeBAG基因为基因工程提供了有价值的目标,以提高激情果种植中的耐热性.
相关概念视频
02:39Gene Flow
37.5K
Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
37.5K
02:13Overview of Transposition and Recombination
18.8K
Transposons make up a significant part of genomes of various organisms. Therefore, it is believed that transposition played a major evolutionary role in speciation by changing genome sizes and modifying gene expression patterns. For example, in bacteria, transposition can lead to conferring antibiotic resistance. Movement of transposable elements within the genetic pool of pathogenic bacteria can aid in transfer of antibiotic-resistant genetic elements. In eukaryotes, transposons can carry out...
18.8K
01:21Frequency-dependent Selection
23.1K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
23.1K
02:05Gene Evolution - Fast or Slow?
8.0K
The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
In contrast, regions which code...
8.0K
02:05Gene Evolution - Fast or Slow?
3.4K
3.4K
02:02Cis-regulatory Sequences
11.6K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.6K