相关实验视频

Updated: May 5, 2026

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Dissecting Host-virus Interaction in Lytic Replication of a Model Herpesvirus

Published on: October 7, 2011

非洲猪瘟病毒MGF 360-2L 破坏宿主抗病毒免疫力基于转录基因分析

Taoqing Zhang^1,2, Xiaodong Qin^1,2, Sujie Dong^1,2

¹State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.

|September 27, 2025

概括

非洲猪瘟病毒MGF 360-2L是一种晚期基因,对调节病毒蛋白表达和宿主免疫反应至关重要. 它的缺失会破坏复制必需的基因,并影响I型干扰素信号通路.

科学领域:

病毒学病毒学
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

非洲猪瘟病毒 (ASFV) 多基因家族 (MGF) 360种蛋白质是免疫逃避,复制和毒性的关键.
许多ASFV MGF蛋白的特定功能仍然不完全理解.

研究的目的:

为了研究ASFV MGF 360-2L蛋白的功能作用.
阐明MGF 360-2L对病毒基因表达和宿主免疫反应的影响.

主要方法:

转录动力学分析以确定MGF 360-2L表达时间.
缺少MGF 360-2L (ΔMGF 360-2L) 的ASFV感染细胞的转录组分析.
基因本体学 (GO) 和KEGG路径对差异表达基因 (DEGs) 的丰富分析.

主要成果:

MGF 360-2L表现出晚期基因表达动力学,类似于p72基因 (B646L).
缺少MGF 360-2L导致感染后12小时和24小时显著的基因表达差异.
DEGs主要丰富于I型干扰素 (IFN-I) 信号通路.
MGF 360-2L对于复制相关基因E199L和E301R的稳定表达至关重要.

结论:

ASFV MGF 360-2L作为一个晚起作用的基因.
MGF 360-2L在精确调节病毒蛋白合成方面发挥着至关重要的作用.
这种蛋白质在ASFV感染期间调节宿主的免疫反应.

关键词:

非洲猪瘟病毒病毒在 MGF 360-2L 中.在RNA-Seqq.宿主抗病毒免疫力宿主抗病毒免疫力重组病毒的功能分析.

更多相关视频

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production

Published on: March 2, 2014

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

相关概念视频

Inhibitors of Viral Protein Synthesis

Inhibitors of Viral Protein Synthesis

Protein synthesis is indispensable for viral replication, as viruses lack the cellular machinery required for this process and must hijack the host's translational apparatus. In response, host cells deploy a critical innate immune defense involving interferons, specialized cytokines that play a central role in inhibiting viral propagation.Upon viral detection, infected cells release interferons that bind to receptors on adjacent uninfected cells, activating the JAK-STAT signaling pathway and...