戈西波尔暴露对卵巢储备功能的影响:基于TRAEC战略的综合风险评估
Xiaoyan Sun1, Jia Ying2, Xuan Ma3
1Center of Reproduction and Genetics, State Key Laboratory of Reproductive Medicine and Offspring Health, the Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou 215000, China.
Toxics
|September 27, 2025
概括
暴露于gossypol会损害雌性小鼠的卵巢储备和卵子质量,导致生育能力下降. 虽然在停止暴露后会出现一些恢复,但内分泌功能仍然受损,将其归类为中度生殖毒素.
科学领域:
- 生殖毒理学 生殖毒理学
- 环境健康 环境健康
- 卵巢生物学 卵巢生物学
背景情况:
- 在棉花种子中发现的一种化合物戈西波尔 (Gossypol) 有已知的毒理作用.
- 了解其对女性生殖的影响对于风险评估至关重要.
研究的目的:
- 评估gossypol在雌性ICR小鼠中的生殖毒性和可逆性.
- 评估gossypol对卵巢储备,卵细胞成熟和细胞亡的影响.
- 为了确定受gossypol暴露影响的分子通路.
主要方法:
- 雌性ICR小鼠被用gossypol (20 mg/kg/天30天) 治疗.
- 进行了卵巢组织学,卵细胞成熟试验和KGN细胞亡试验.
- 蛋白质基因分析被用来识别差异表达的蛋白质.
- 应用了环境化学品有针对性的风险评估 (TRAEC) 框架.
主要成果:
- 戈西波尔治疗导致体重减轻,原始卵泡枯竭,卵细胞成熟受损.
- 在KGN细胞中观察到显著的亡增加.
- 蛋白质组分析显示了类固醇生成,免疫调节和线粒体代谢的改变.
- 戒断后,观察到部分形态恢复,但内分泌功能仍然受到损害.
结论:
- 戈西波尔在雌性小鼠中显著损害卵巢储备和卵细胞能力.
- 戈西波尔的生殖毒性仅显示部分可逆性.
- 戈西波尔被归类为中度生殖毒性物质,在临床应用中需要谨慎.
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