利科弗拉B通过向病毒RNA依赖RNA聚合酶 (RdRp) 来抑制流感A病毒
Pu Fan1,2, Peng Lv2, Sen Zhang3
1School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China.
Viruses
|September 27, 2025
概括
来自甘的利科夫拉B,通过向病毒RNA聚合酶,对抗流感A和B病毒表现出强大的广泛抗病毒活性. 这种天然化合物为开发有效的流感治疗提供了一个有希望的新途径.
科学领域:
- 病毒学 病毒学
- 药理学 药理学是指药理学的学科.
- 自然产品化学 自然产品化学
背景情况:
- 流感大流行是全球健康面临的重大挑战,因抗病毒耐药性增加而加剧.
- 传统中国医学使用甘 (Glycyrrhiza spp.) 对于呼吸道疾病,其化合物已经显示出抗SARS-CoV-2活性.
- 对于抗击流感的新型,广泛的抗病毒药物有着至关重要的需求.
研究的目的:
- 为了识别和描述具有强大的抗流感活性的天然化合物.
- 为了研究黄素B对流感病毒的作用机制.
- 评估利科弗拉B的广泛疗效和治疗潜力.
主要方法:
- 来自甘 (Glycyrrhiza spp.) 的黄素B的鉴定 ) 的情况.
- 在体外抗病毒测定针对各种流感A和B菌株.
- 机理学研究包括病毒RNA依赖RNA聚合酶 (RdRp) 向,分子对接,西部斑点和免疫光分析.
- 选择性指数 (SI) 的确定.
主要成果:
- 利科夫拉B显示出对多种流感A亚型和流感B病毒的强有力的抑制作用.
- 该化合物选择性地向病毒RdRp,抑制病毒复制,具有有利的SI (14.9-29.9).
- 分子对接和实验验证证证实了与RdRp子单元的相互作用和减少病毒蛋白表达.
- 证实了针对H1N1,H3N2和B型流感的临床隔离品的广谱活性.
结论:
- 利科弗拉B是各种流感病毒的强有力的天然抑制剂.
- 它的机制涉及选择性向病毒RdRp的机制.
- 利科弗拉B代表了一种有前途的化合物,用于开发新型,广泛的抗病毒疗法来对抗流感.
更多相关视频
06:28Immunofluorescence to Monitor the Cellular Uptake of Human Lactoferrin and its Associated Antiviral Activity Against the Hepatitis C Virus
Published on: October 1, 2015
14.0K
09:31Fluorescence-based Neuraminidase Inhibition Assay to Assess the Susceptibility of Influenza Viruses to The Neuraminidase Inhibitor Class of Antivirals
Published on: April 15, 2017
19.0K
相关概念视频
Leaky Scanning
5.6K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.6K
Experimental RNAi
7.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
7.3K
RNA Interference
27.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
27.8K
Viruses with RNA Genomes
824
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
824
Transcriptional Regulation: Riboswitches
588
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
588
Types of RNA
72.5K
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.5K
