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相关概念视频

Exon Recombination02:32

Exon Recombination

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The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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A Murine Model of Dengue Virus-induced Acute Viral Encephalitis-like Disease
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整合机制研究确定了Reticulon-3作为传染性外体驱动的登革热病原体的关键调节器.

Razieh Bitazar1,2, Clinton Njinju Asaba1, Saina Shegefti1

  • 1Armand-Frappier Santé Biotechnologie Research Center, Institut National de la Recherche Scientifique, Laval, QC H7V 1B7, Canada.

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概括

登革热病毒 (DENV) 使用Reticulon 3异型S (RTN3S) 来创建传染性外体,以便传播. RTN3S对于病毒释放和免疫逃避至关重要,为抗病毒疗法提供了一个新的目标.

关键词:
抗病毒药物 抗病毒药物登革热:登革热是指登革热的一种疾病.这是一种内塑的内塑.弗拉维病毒 (flavivirus) 是一种病毒.免疫逃生 免疫逃生传染性外基因组中的感染性外基因组.单细胞 (Monocytes) 是一种单细胞.多种多样性的多种类型的.病变的发生和发病.网球 3 3 网球走私和贩运的人口贩运

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科学领域:

  • 病毒学 病毒学
  • 细胞生物学 细胞生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 登革热病毒 (DENV) 利用宿主外体通路进行传播和免疫逃避.
  • 促进DENV外因子生物发生的宿主因素尚未得到充分理解.

研究的目的:

  • 研究宿主因子在DENV介导的外体细胞生成和病毒释放中的作用.
  • 为了确定DENV病变的新机制和潜在的治疗点.

主要方法:

  • 在DENV感染的肝细胞和单细胞细胞中研究了Reticulon 3异型S (RTN3S) 表达.
  • 评估了RTN3S与病毒组件 (dsRNA,NS3) 的物理关联.
  • 在RTN3S操纵 (敲击/过度表达) 后,量化了外体细胞的产生和病毒的输出.
  • 在DENV感染细胞和患者样本中分析了单细胞表型 (CD16表达) 和细胞因子分泌.

主要成果:

  • DENV感染诱导RTN3S表达,该表达会与病毒RNA和NS3结合.
  • RTN3S对于高效的外体细胞生成和传染性病毒颗粒的释放至关重要.
  • 过度表达RTN3S增强了感染性外体释放,这取决于其C端域.
  • 被DENV感染的单细胞表现出改变的CD16表达,高调的囊泡贩运基因,以及增加的炎症性细胞因子分泌.
  • 来自登革热患者的单细胞表现出高RTN3表达与CD16+亚群扩张和囊泡机械基因丰富相关.

结论:

  • DENV劫持RTN3S以促进传染性外体的形成,促进病毒传播和免疫逃避.
  • RTN3S是DENV病变的关键宿主因素,也是宿主导的抗病毒策略的潜在目标.