预测的结构Ceduovirus粘附装置突出显示独特的架构,让人想起细菌分泌系统VI
Adeline Goulet1, Jennifer Mahony2, Douwe van Sinderen2
1Laboratoire d'Ingénierie des Systèmes Macromoléculaires (LISM), Institut de Microbiologie, Bioénergies et Biotechnologie (IMM), Aix-Marseille Université-CNRS, UMR 7255, 13009 Marseille, France.
Viruses
|September 27, 2025
概括
感染乳酸细菌的菌体具有独特的尾部结构. AlphaFold3揭示了对Ceduovirus菌体粘附装置的新见解,有助于基因注释和理解宿主相互作用.
科学领域:
- 微生物学 微生物学
- 结构生物学 结构生物学
- 生物信息学是一种生物信息学.
背景情况:
- 菌体 (菌体) 是影响工业发酵的重要细菌病毒.
- 乳酸细菌 (LAB) 菌体,特别是细菌病毒,造成重大经济损失.
- 感染Lactococcus lactis的Ceduovirus菌体的宿主粘附装置 (HADs) 了解得还不够.
研究的目的:
- 使用AlphaFold3.3阐明Ceduovirus HADs的结构和功能.
- 为了改善Ceduovirus结构基因的注释.
- 了解Ceduovirus-Lactococcus lactis相互作用的分子机制.
主要方法:
- 利用AlphaFold3进行了体结构预测,用于预测Ceduovirus HADs.
- 对已知的蛋白质系统的功能洞察和相似性分析了预测的结构.
- 杆结构预测用于比较基因组学和基因注释.
主要成果:
- 病毒HADs表现出不同于其他感染格拉姆阳性细菌的菌体的新奇特征.
- 这些HAD与细菌分泌系统VI具有结构同质性.
- 这些HAD具有糖和蛋白质结合的能力.
- 大多数ceduovirus结构基因都成功地根据预测的结构进行了注释.
结论:
- AlphaFold3显著提升了对Ceduovirus菌体生物学和基因组注释的理解.
- 病毒HADs代表了一类独特的菌体粘附系统,具有双重结合能力.
- 这项研究为开发基于菌体的策略提供了基础,以保护LAB发酵过程.
相关概念视频
Structure of Cadherins
4.7K
The cadherins were one of the first cell adhesion molecules discovered; the term “cadherins” is based on their calcium-dependent adhering properties. The first cadherins discovered on the epithelial, neuronal, and placental cells were named E-cadherin, P-cadherin, and N-cadherin, respectively. These classical cadherins share sequence and structural similarities. Other cadherins, including those involved in cell signaling, are grouped into non-classical cadherins. This...
4.7K
Adherens Junctions
6.2K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
6.2K
Viral Structure
73.8K
Viruses are extraordinarily diverse in shape and size, but they all have several structural features in common. All viruses have a core that contains a DNA- or RNA-based genome. The core is surrounded by a protective coat of proteins called the capsid. The capsid is composed of subunits called capsomeres. The capsid and genome-containing core are together known as the nucleocapsid.
73.8K
Clathrin Coated Vesicles
9.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.0K
Fimbriae, Pili, and Axial Filaments
1.6K
Fimbriae and pili are specialized bacterial surface structures that play pivotal roles in adhesion, genetic exchange, and motility. Composed primarily of pilin protein, these hairlike appendages are crucial for bacterial survival and pathogenicity in various environments.Fimbriae: Adhesion and PathogenicityFimbriae are fine, filamentous structures measuring 2–10 nanometers in diameter and are densely distributed on the bacterial cell surface. They facilitate bacterial adhesion to abiotic...
1.6K
Immunoglobulin-like Cell Adhesion Molecules
4.2K
Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
4.2K


