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该属的全基因组变异概况托巴莫病毒

Amany E Gomaa^1,2, Hernan Garcia-Ruiz¹

¹Department of Plant Pathology and Nebraska Center for Virology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.

|September 27, 2025

概括

托巴莫病毒呈现低基因组变异,并且处于负选择状态. 然而,新出现的番茄棕色果病毒 (ToBRFV) 显示了持续的进化,受地理和宿主的影响,影响了番茄生产.

科学领域:

植物病毒学植物病毒学
分子进化分子进化
基因组学就是基因组学.

背景情况:

托巴莫病毒 (Tobamovirus) 是维尔加病毒 (Virgaviridae) 科中的一个属,具有具有四个开放的读取框架的正义单链RNA基因组.
传染发生在机械和通过种子.
在2015年发现的番茄棕色果病毒 (ToBRFV) 已经克服了番茄的耐药性,造成了全球重大损失.

研究的目的:

描述整个托巴莫病毒基因组中的核酸变异.
分析每个开放的读取框架上的选择压力.
为了比较ToBRFV和相关物种之间的基因组多样性,例如番茄马赛克病毒 (ToMV) 和烟草马赛克病毒 (TMV).

主要方法:

全基因组核酸变异分析.
在开放的阅读框架上进行选择压力评估.
遗传学和多样性分析.

主要成果:

托巴莫病毒通常具有较低的核酸多样性,并且在所有开放的读取框架中处于负选择之下.
没有发现过度可变的基因组区域.
在ToBRFV,ToMV和TMV中的运动蛋白区域显示出较低的核酸多样性,尽管它含有破坏抗性的突变.
遗传学分析表明ToBRFV正在进行演变,地理分布和宿主因素塑造了其基因组多样性.

结论:

托巴莫病毒通常是保存的,负选择对它们的基因组起作用.
ToBRFV表现出持续的进化,对番茄作物构成持续的威胁.
基因组多样性受到地理分布和宿主相互作用的影响,需要持续监测.

关键词:

在ToBRFVV中.托巴莫病毒 (Tobamovirus) 是一种病毒.托巴莫病毒的果实和.核酸多样性的核酸多样性遗传学分析遗传学分析阻力破裂阻力破裂选择压力选择压力

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