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对于子大小变异的不同选择影响了与胚胎发育和生存相关的卵管外细胞囊中的RNA载荷

Carmen Almiñana^1,2, María-José Argente³, Daniel Serrano-Jara³

¹Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Lindau, Zurich, Switzerland.

Biological research

|September 27, 2025

概括

与高变异线相比,为低 litter 尺寸变异选择的子线显示出明显的卵管外细胞囊泡 (EV) RNA 载荷. 卵巢内EVs (oEVs) 的这些差异可能解释早期胚胎发育和孕产妇支持的变化.

科学领域:

生殖生物学生殖生物学
动物遗传学动物遗传学
细胞外囊泡中的细胞外囊泡.

背景情况:

选择均的 litter 尺寸旨在增强动物的压力和疾病抵抗力.
为了减少 litter 尺寸变化而选择的线条 (L 线条) 显示出较高的 litter 尺寸,而不是为增加变化而选择的线条 (H 线条),可能是由于卵巢胚胎存活率的改善.
卵巢外细胞囊泡 (oEVs) 对于早期胚胎发育至关重要,但它们在子中的作用仍未得到充分研究.

研究的目的:

为了研究子卵管环境对子大小变化的选择的影响.
分析卵管外细胞囊 (oEVs) 和它们的RNA载荷对不同线胚胎发育的贡献.

主要方法:

从H和L线收集孕妇 (P) 和非孕妇 (NO) 的卵巢液.
排卵管EVs (oEVs) 被隔离和表征.
在oEV上进行RNA测序,以分析群体之间的基因差异和miRNA表达.

主要成果:

在每一线的孕妇和非孕妇之间观察到差异丰富 (DA) 基因和miRNA的显著差异.
功能性丰富分析显示,与胚胎发育和形态发生相关的术语与L线具有独特的关联.
虽然在非怀孕的母狗中发现了169个DARNA在H和L线之间,但在怀孕的母狗中发现的RNA或miRNA载荷之间没有显著的差异.

结论:

这项研究是首次揭示了针对低与高 litter 尺寸变化选择的子线之间的明显 oEV RNA 货物配置文件.
在oEVs中蛋白质编码RNA和miRNA载荷的观察到的差异表明,在线条之间对早期胚胎发育提供不同程度的母体支持.

关键词:

不同的选择分离选择.没有人知道,没有人知道.胚胎胚胎是一个胚胎.细胞外囊泡中的细胞外囊泡.垃圾大小垃圾大小垃圾大小一个被发现的怪物.怀孕怀孕在怀孕怀孕通过RNA测序进行RNA测序.一个子子.

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