热纳斯蒂克益生菌工程生物材料,动态响应炎症标记物
Gokce Altin-Yavuzarslan1,2,3, Sierra M Brooks4, James O Park5
1Molecular Engineering and Sciences Institute, University of Washington, 3946 W Stevens Wy NE, Seattle, WA, 98195, USA.
使用3D打印的工程生物材料 (ELM) 提供智能益生菌来对抗炎症性肠病 (IBD). 这些设备检测炎症并产生抗炎化合物,提供一种新的治疗方法.
科学领域:
- 生物材料工程 生物材料工程
- 合成生物学 合成生物学
- 胃肠病学 胃肠病学
背景情况:
- 传统的炎症性肠病 (IBD) 治疗有其局限性.
- 工程益生菌提供有针对性的治疗化合物生产.
- 有效的传递系统对于工程化益生菌的生存能力和局部化至关重要.
研究的目的:
- 开发3D打印的工程生物材料 (ELM) 用于局部炎症治疗.
- 设计益生菌生物来感知炎症生物标志物并产生抗炎剂.
- 为了在体外评估ELM设备的疗效.
主要方法:
- 使用基因电路的工程化大肠杆菌Nissle 1917.
- 将细菌纳入聚乙烯甘二烯酸盐 (PEGDA) 树脂中.
- 采用基于光的3D打印来创建ELM结构.
- 评估了氧化检测和抗炎化合物生产.
- 在小鼠巨细胞和肠上皮细胞模型中测试了ELM疗效.
主要成果:
- 成功地3D打印了可行的和代谢活跃的ELM设备.
- ELM检测到氧化,这是一个关键的炎症生物标志物.
- 在对炎症的反应中,ELM产生的三胺和1-乙-3-碳酸-β-碳酸.
- 在体外炎症模型中治疗炎症的有效性已被证明.
结论:
- 3D打印的ELM显示出局部化,按需治疗IBD的前景.
- 这些神经治疗器件在传统疗法上提供了潜在的进步.
- 进一步开发可能会导致炎症性疾病的长期管理.
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