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两个伊萨蒂斯基因组揭示了植物的生物合成和进化起源

Junfeng Chen^1,2, Hexin Tan³, Jun Yang⁴

¹The SATCM Key Laboratory for New Resources & Quality Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.

Genomics, proteomics & bioinformatics

|September 27, 2025

概括

研究人员在伊萨蒂斯植物中发现了印花染料难以捉摸的生物合成途径. 他们确定了关键酶,含有黄素的单氧化酶 (FMO) 和细胞P450 (CYP) 蛋白质,揭示了植物中的代谢创新.

科学领域:

植物生物化学植物生物化学
进化基因组学是进化的基因组学.
代谢途径分析

背景情况:

色染料,历史上来源于Isatis属,具有很长的人类使用历史.
伊萨蒂斯的印花生产的特定生物合成途径仍然是未知的.
之前的研究表明,一些Isatis种类已经失去了生产印花的能力.

研究的目的:

为了阐明伊萨蒂斯属的生物合成途径.
研究蓝生物合成的进化起源和机制.
识别参与印花生产的关键酶和基因.

主要方法:

对伊萨蒂斯种群的遗传学和代谢分析.
在Isatis indigotica和Isatis cappadocica的新基因组测序,组装和比较基因组学.
在Nicotiana benthamiana中同源的基因表达用于识别酶功能.

主要成果:

在伊萨蒂斯种群中,印花生产能力各不相同,有些人已经失去了这种能力.
蓝生物合成中的关键氧化步骤是由含有黄素的单氧化酶 (FMO) 和细胞染色体P450 (CYP) 酶催化.
有证据表明,这种代谢创新源于植物的氧化物通路.

结论:

这项研究揭示了印花生物合成在Isatis的遗传基础和进化轨迹.
对CYP和FMO酶的新功能化在印花生产的进化中起到了至关重要的作用.
这些发现为植物代谢途径的演变提供了新的见解.

关键词:

在CYP79B2B2中.弗拉文单氧基酶的使用属伊萨蒂斯属伊萨蒂斯属蓝生物合成的生物合成氧化物通路的演变过程

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