Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

8.3K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
8.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Base editing reveals an essential role for NANOG in human embryogenesis.

Nature·2026
Same author

Efficient prime editing in vivo and in vitro using lipid nanoparticles.

Nature nanotechnology·2026
Same author

Peptide-MHC-targeted engineered virus-like particles enable selective priming and gene editing of tumor-specific T cells.

Cell reports·2026
Same author

Anti-CRISPR-mediated continuous directed evolution of CRISPR-Cas9 in human cells.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

AI-guided redesign of laboratory-evolved reverse transcriptases enhances prime editing.

Nature biotechnology·2026
Same author

Synonymous editing alters ion channel function, favoring prime editing for retinal disease correction.

International journal of biological sciences·2026

相关实验视频

Updated: Jan 16, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K

超微小的化学遗传标签与组转移带.

Vedagopuram Sreekanth1, Shaimaa H Sindi1, Santosh K Chaudhary1

  • 1Chemical Biology and Therapeutics Science, Broad Institute of MIT and Harvard, Cambridge, MA, 02142, USA.

Angewandte Chemie (International ed. in English)
|September 27, 2025
PubMed
概括

研究人员开发了超小化学遗传标签 (mgTag和cTag) 来研究蛋白质. 这些新的标签是现有的最小的标签,为生物技术和医学带来了新的可能性.

关键词:
这是一个C1域名.化学遗传标签 化学遗传标签诱导的邻近性诱导的邻近性在KRAS信号传输中.分子粘合剂是一种分子粘合剂.

更多相关视频

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.7K
Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
10:10

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

11.3K

相关实验视频

Last Updated: Jan 16, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

9.0K
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.7K
Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags
10:10

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

11.3K

科学领域:

  • 生物化学 生物化学
  • 分子生物学分子生物学
  • 化学生物学 化学生物学

背景情况:

  • 化学遗传标签对于研究蛋白质功能至关重要,当小分子配体无法使用时.
  • 现有的标签往往太大,阻碍了它们与某些感兴趣的蛋白质 (POI) 的应用.

研究的目的:

  • 为更广泛的POI应用开发和表征新的,超微小的化学遗传标签.
  • 为了证明这些标签在维持蛋白质功能和诱导近距离的实用性.

主要方法:

  • 设计了两个超小化学遗传标签:mgTag (36 aa) 和cTag (50 aa).
  • 利用转移酶类型的反应性用于连接器的附着.
  • 评估标签对KRAS (G12D) 信号通路的影响.
  • 使用标记蛋白质研究了阿贝尔森酶 (ABL) 和BRD4之间的近距离诱导.

主要成果:

  • mgTag和cTag是报告中最小的化学遗传标签.
  • 与较大的标签不同,mgTag或cTag与KRAS ((G12D) 的融合并没有破坏其生长信号通路.
  • 一个BRD4结合物的组转移到被标记的ABL诱导了BRD4的接近和酸化.
  • 当移转酶类型的反应性被删除时,观察到酸化的减少.

结论:

  • 超小型化学遗传标签 (mgTag,cTag) 克服了较大的标签的局限性.
  • 这些标签可以进行功能研究和近距离诱导信号.
  • 集团转移机制为设计近距离诱导的嵌合体提供了一种有效的策略.
  • 这些标签在基础科学,生物技术和医学方面具有广泛的潜在应用.