由Ophiorrhiza mungos L L的多种内菌微生物产生的坎普托他的定量变化
Mary Theresa1, Arya Nikathil Pradeep1, Aswani Ravi2,3
1School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
Biotechnology progress
|September 27, 2025
概括
来自Ophiorrhiza mungos植物的内菌微生物被选为抗癌化合物坎普托西因 (CPT) 的生产. 十七个菌株,主要是细菌,产生了CPT,突出显示了可持续CPT制造的新型微生物来源.
科学领域:
- 微生物学 微生物学
- 自然产品化学 自然产品化学
- 生物技术是生物技术.
背景情况:
- 药用植物含有内性微生物 (EMs),可以产生有价值的生物活性化合物.
- 坎普托西因 (CPT) 是一种强效的抗癌药物,其治疗需求很高,需要可持续的生产方法.
- 菌 (Ophiorrhiza mungos) 是已知的CPT来源,但其内性微生物群落对于CPT生物合成还未得到充分探索.
研究的目的:
- 为了研究从Ophiorrhiza mungos中分离出的内菌微生物的CPT生物合成潜力.
- 通过选择的细菌和真菌内细胞识别和量化CPT产量.
- 探索新的微生物来源,以实现可持续和经济高效的CPT生产.
主要方法:
- 从Ophiorrhiza mungos.的各个部分分离和培养了175种内菌微生物.
- 使用高性能液态染色学 (HPLC) 进行CPT生产的选.
- 通过液体染色学-并联质谱法 (LC-MS/MS) 确认和量化CPT.
主要成果:
- 17种内菌菌株 (14种细菌,3种真菌) 显示出CPT的产生,其中大多数是从根组织中分离出来的.
- 阿尔卡利格尼斯 (Alcaligenes faecalis) 亚种. 类S18产生了最高的CPT,为1294.52μg/L,其次是Bacillus tequilensis (309.02μg/L).
- 这项研究报告了Alcaligenes faecalis,Aspergillus sp.的CPT生产情况,并首次记录了其在Stenotrophomonas,Fictibacillus,Acinetobacter和Pseudomonas属中的合成情况.
结论:
- 来自Ophiorrhiza mungos的内菌微生物代表了CPT生产的重要储备.
- 新型细菌和真菌菌株,特别是Alcaligenes faecalis和Aspergillus sp.,为CPT提供了有前途的产量.
- 这些发现强调了微生物内细胞作为商业CPT制造的可持续和经济有效的替代品的潜力.
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