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Updated: Jul 10, 2026

Laser Microirradiation to Study In Vivo Cellular Responses to Simple and Complex DNA Damage
10:44

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Published on: January 31, 2018

通过活细胞成像来量化PARP1动态和活性中PARP抑制剂诱导的变化的协议

Petar-Bogomil Kanev1, Sylvia Varhoshkova1, Aleksander Aleksandrov2

  • 1Laboratory of Genomic Stability, Institute of Molecular Biology, Bulgarian Academy of Sciences, Acad. G. Bonchev Str. Bl.21, 1113 Sofia, Bulgaria.

STAR protocols
|September 27, 2025
PubMed
概括

这项研究引入了一种活细胞成像方法,用于追踪PARP1和PAR等DNA修复蛋白. 该协议可视化了DNA损伤部位的蛋白质动态,有助于研究DNA修复药物机制.

关键词:
生物物理学的生物物理.癌症 癌症 癌症 癌症细胞生物学 细胞生物学细胞培养培养的细胞培养.基于细胞的测试显微镜的使用方法分子生物学分子生物学

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...