相关实验视频

Updated: Jul 22, 2026

A Universal Protocol for Large-scale gRNA Library Production from any DNA Source
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A Universal Protocol for Large-scale gRNA Library Production from any DNA Source

Published on: December 6, 2017

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简化并行干扰的协议使用简化长非编码RNACRISPR库简化并行干扰.

Joshua M Hazan1, Nisrine Lahoud-Jeries1, Assaf C Bester1

  • 1Technion-Israel Institute of Technology, Faculty of Biology, Technion City, Haifa 3200003, Israel.

STAR protocols
|September 27, 2025
PubMed
概括

这项研究引入了针对长非编码RNA (lncRNAs) 的CRISPR干扰 (CRISPRi) 屏幕的新协议. 这种方法可以使用专门的sgRNA库有效地发现功能性lncRNA.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?细胞生物学 细胞生物学基因表达 基因表达遗传学 是一个遗传学.

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相关概念视频

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...