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转酶PagTAL1调节ROS恒温,以改善Populus中的热应激耐受性

Xinyuan Wu¹, Yufen Bu¹, Cheng Pan¹

¹State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Technology, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China; National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China; The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, No. 35 Qinghua East Road, Beijing, 100083, China.

Plant physiology and biochemistry : PPB

|September 27, 2025

概括

这是一棵树.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
生物化学生物化学

背景情况:

高温会影响植物生长.
转酶 (TAL) 对于植物生长至关重要.
木对热应激的反应尚未完全理解.

研究的目的:

描述树转酶基因的特征,PagtAL1.1.
研究PagTAL1在耐热性中的作用.
确定 PagTAL1.1 的监管机制.

主要方法:

基因鉴定和特征化.
组织特异性表达分析.
代谢概况. 代谢概况.
在热应激下进行表型分析.
在CRISPR/Cas9基因编辑中.
双露西法酶测定.双露西法酶测定.

主要成果:

PagTAL1局限于叶绿体,并表现出差异性表达.
通过ROS清理,PagTAL1可以增强耐热性.
过度表达提高了耐热性;淘汰会增加灵敏度.
PagMYB94 直接激活了 PagTAL1 的表达.

结论:

帕格塔尔1是木耐热性的关键调节剂.
通过ROS清理,PagTAL1可以减轻热应激.
PagTAL1是工程耐热树的潜在目标.

关键词:

这就是CRISPR/Cas9的作用.页面MYB94 的页面第1页第1页第1页这就是ROSOS ROS.耐热性耐热性耐热性转基因的转基因

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