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进化分析和免疫诱导的表达和m⁶ 一个修改的RIPK1和RIPK2在米的croaker

Qianru Xing¹, Shang Geng¹, Xing Lv¹

¹Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.

Fish & shellfish immunology

|September 27, 2025

概括

这项研究表明,N6-甲基氨酸 (m⁶A) 修饰调节受体相互作用蛋白激酶 (RIPK1和RIPK2) 在 miiuy croaker. 这些表观遗传变化会影响鱼类对炎症刺激的免疫反应.

科学领域:

免疫学免疫学免疫学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.
分子生物学分子生物学

背景情况:

受体相互作用蛋白激酶 (RIPKs),包括RIPK1和RIPK2,对于炎症信号和细胞死亡至关重要.
在调节RIPK中N6-甲基氨酸 (m⁶A) 修饰的作用尚不清楚.
了解RIPK调节对于鱼类天生的免疫力至关重要.

研究的目的:

为了识别和表征Miichthys miiuy (Miichthys miiuy) 中RIPK1和RIPK2基因.
研究m⁶A修饰在RIPK1和RIPK2表达的调节中的作用.
探索m6A对鱼类免疫反应的影响.

主要方法:

用于基因识别,进化保存和结构特征的生物信息分析.
甲基化RNA免疫沉降测序 (MeRIP-seq) 和MeRIP-PCR用于检测m6A丰富.
在用聚I:C和脂聚糖 (LPS) 刺激后进行基因表达分析.

主要成果:

在 miiuy croaker 中确定了 RIPK1 和 RIPK2 基因,显示了保存的结构和潜在的免疫功能.
在RIPK1和RIPK2的停止编码子附近发现了显著的m6A丰富.
在poly (I:C) 和LPS刺激后,RIPK1和RIPK2的表达增加.
聚I:C增加了RIPK1m6A水平,而LPS降低了RIPK2m6A水平.
甲基化抑制增加了RIPK1和RIPK2的表达.

结论:

m⁶ 一种修饰在微调涉及RIPK1和RIPK2的免疫调节网络中起作用.
通过m6A的表观遗传调节会影响RIPK的表达动态,以应对免疫刺激.
这项研究提供了对鱼类天生的免疫力和RIPK家族调节的m⁶A介导控制的见解.

关键词:

进化进化进化进化进化进化进化免疫刺激免疫刺激孩子们的孩子们的孩子.在RIPK1中使用.在RIPK2中使用RIPK2.m(6) A6) 一个

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