检查环境水中的固相提取方法的化学空间覆盖面,以便使用LC-MS非向分析方法
Laura D Brunelle1, Eva K Stebel2, Angela L Batt2
1Oak Ridge Institute for Science and Education (ORISE) Participant at the U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr, Cincinnati, OH, 45268, United States.
The Science of the total environment
|September 28, 2025
概括
研究人员优化了四种固相提取 (SPE) 方法与液体染色学高分辨率质谱学 (LC-HRMS) 结合,用于环境水分析. 这些方法提高了化学空间的特征,并改善了各种污染物的非目标分析结果.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 在环境监测中,非定向分析 (NTA) 需要强大的样本准备,以捕获广泛的化学化合物.
- 从NTA中解释复杂的化学混合物是具有挑战性的,因为被捕获的巨大化学空间.
- 固相提取 (SPE) 是在仪器分析之前缩和净化分析物的关键技术.
研究的目的:
- 探索和优化多重固相提取 (SPE) 方法,以在非目标分析 (NTA) 中增强化学空间表征.
- 评估各种SPE化学品的提取效率,用于各种替代化学品.
- 开发和描述最优化的SPE方法,适用于未来环境水样的NTA.
主要方法:
- 研究了多种SPE弹化学成分 (HLB,WAX,MCX,GCB,WCX) 和组合.
- 使用231个替代标准在实验室和环境表面水中评估提取效率.
- 使用液体色谱与高分辨率质谱 (LC-HRMS) 结合用于化学特征检测和分析.
主要成果:
- 四种优化的SPE方法 (HLB,HLB-WAX,HLB-MCX,HLB-WAX-MCX) 证明了各种化学类的有效保留.
- 在HLB-WAX-MCX方法中,保留了最多的替代化学物质 (221种中222种).
- 分析揭示了捕获的显著化学特征,并观察到MCX含有的方法在保留极性离子中的好处.
结论:
- 优化的SPE方法提供了更好的化学空间覆盖,并有助于解释NTA结果.
- 开发的方法提供了不同的化学保留能力,满足不同的NTA目标.
- 这些优化的SPE协议为未来的环境水NTA努力提供了宝贵的工具.
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