用转录组数据集成对外围血液中性粒细胞进行蛋白质分析,揭示了结核病感染诊断的生物标志物
Jiarong Yang1, Zizheng Lv2, Liguo Liu1
1NHC Key Laboratory of Systems Biology of Pathogens, National Institute of Pathogen Biology, and Center for Tuberculosis Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, China.
使用三种中性粒细胞蛋白 (B2M,TXN,PRDX5) 的新诊断测试显示,在检测Mycobacterium tuberculosis (MTB) 感染方面具有很高的准确性,有助于结核病的诊断.
科学领域:
- 生物化学 生物化学
- 免疫学 免疫学 免疫学
- 传染性疾病 传染性疾病
背景情况:
- 结核病 (TB) 仍然是全球主要的健康威胁,需要改进诊断工具.
- 目前的结核病诊断在速度和准确性方面存在局限性,这阻碍了有效的控制工作.
- 中性粒细胞是结核病感染中的关键免疫细胞,也是诊断生物标志物的潜在来源.
研究的目的:
- 描述活跃结核病 (ATB),潜伏结核病感染 (LTBI) 和健康对照 (HC) 中的中性粒细胞蛋白质概况.
- 在与MTB感染相关的中性粒细胞中识别差异表达蛋白 (DEPs).
- 验证候选蛋白质生物标志物用于诊断MTB感染.
主要方法:
- 对中性粒细胞蛋白质谱的横截面蛋白质组分析.
- 使用严格标准识别DEP,并与转录基因数据进行整合.
- 在319个个体的队列中,使用自动化西部涂抹验证三个DEP (B2M,TXN,PRDX5) 作为生物标志物.
主要成果:
- 在ATB,LTBI和HC组中发现了数百个DEP.
- 路径丰富分析显示了NOD类受体信号通路的显著参与.
- 在B2M,TXN和PRDX5的组合下,MTB感染的诊断性能高 (AUC=0.9847,敏感性=95.11%,特异性=96.23%).
结论:
- 这项研究提供了在不同MTB感染状态中中性粒细胞的全面蛋白质组景观.
- 鉴定的三种蛋白质签名 (B2M,TXN,PRDX5) 显示出作为MTB感染的新型诊断生物标志物的潜力.
- 这种生物标志物组合可以显著帮助准确和快速诊断结核病.
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