城市污水处理厂中的功能性抗体对公共卫生构成挑战
Roland Wirth1, Bernadett Pap2, Márk Szuhaj1
1Institute of Plant Biology, HUN-REN Biological Research Centre, Szeged, Hungary; Department of Biotechnology and Microbiology, University of Szeged, Szeged, Hungary.
Water research
|September 28, 2025
概括
污水处理厂 (WWTP) 控制抗菌素耐药性,但也会传播它. 这项研究发现,虽然与人类相关的抗性基因减少,但许多基因仍然活跃,而质粒有助于传播.
科学领域:
- 环境微生物学环境微生物学
- 抗微生物耐药性 抗微生物耐药性
- 基因组学和转录基因组学
背景情况:
- 污水处理厂 (WWTP) 对公共卫生至关重要,但可以隐藏和传播抗生素耐药性基因 (ARG).
- 了解WWTP中的ARG动态对于有效的抗菌素耐药性 (AMR) 控制策略至关重要.
- 微生物群落,治疗过程和ARG持久性之间的相互作用需要详细的研究.
研究的目的:
- 通过综合元基因组学和元转录组学来研究市级WWTP的抗体.
- 分析不同治疗阶段和季节的ARG的丰度和转录活性.
- 为了确定关键的微生物参与者和移动基因元素参与ARG传播在WWTPs.
主要方法:
- 基因组解析的元基因组学和元转录组学应用于来自市政WWTP的样本.
- 分析涵盖了各种治疗阶段和季节性变化.
- 基因组组装基因组 (MAGs) 被构建以识别ARG携带的细菌,并评估它们的转录活性.
主要成果:
- 人类相关的,潜在的致病性ARG载体MAG在治疗期间减少,但许多ARG仍然在转录方面活跃.
- 塑被确定为移动抗性的重要载体,环境微生物占主导地位.
- 季节性变化影响了致病性与环境ARG携带MAG的丰富性;诺基诺隆耐药性基因表达有所变化,特别是在无氧治疗期间更低.
结论:
- 整合基因组和转录组数据为特定地点的ARG动态及其载体提供了宝贵的见解.
- 尽管丰度和表达低,但在潜在的人类病原体中发现了临床相关的ARG,例如Citrobacter freundii.
- 加强对WWTP等工程系统的监测对于控制AMR至关重要,考虑到耐药性的遗传和转录方面.
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