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在登革热病毒感染中通过网络分析识别miRNA-mRNA同表达

Rajesh Das¹, Sasivarman Selvam¹, Vigneshwar Suriya Prakash Sinnarasan¹

¹Department of Bioinformatics, Pondicherry University, Kalapet, Puducherry 605014 India.

|September 29, 2025

概括

这项研究揭示了在登革热病毒 (DENV) 感染期间微RNA (miRNA) 和信使RNA (mRNA) 之间的关键分子相互作用. 这些发现确定了管理DENV感染的潜在诊断标志物和治疗点.

科学领域:

分子生物学分子生物学
病毒学病毒学
基因组学就是基因组学.

背景情况:

登革热病毒 (DENV) 感染对全球健康构成重大挑战,导致从登革热到严重登革热出血热等疾病.
了解DENV感染的分子机制对于开发有效的诊断和治疗至关重要.

研究的目的:

研究涉及DENV感染的复杂miRNA-mRNA调节网络.
为了确定潜在的诊断生物标志物和登革热病毒的治疗点.

主要方法:

来自DENV感染和对照样本的mRNA和miRNA测序数据的综合分析.
差异表达分析以识别改变的基因和miRNAs.
生物信息工具 (miRNet,Metascape) 用于目标基因识别和功能丰富分析.
皮尔森相关性和接收器运行特征 (ROC) 分析以确定监管关系和诊断潜力.

主要成果:

在DENV感染中确定了不同的差异表达基因 (DEGs) 和miRNAs.
上调的基因参与DNA模板转录和细胞分裂;下调的基因参与TNF-alpha信号传递和血管发育.
突出显著的miRNA-mRNA调节对,其中几个对 (例如,TUBA1A-hsa-mir-205-5p) 显示出完美的诊断潜力 (AUC=1).

结论:

该研究阐明了在DENV感染的背景下关键的miRNA-mRNA调节网络.
确定了具有高诊断价值的特定miRNA-mRNA相互作用,为改善登革热诊断提供了潜力.
这些发现为开发针对登革热病毒感染的新型向治疗提供了基础.

关键词:

相关性网络的相关性网络.DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG DEG 登革热病毒感染者这就是米RNA.没有NGS,没有NGS.负相关性是负相关性的.

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MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...