纳米粒子表面化学的异体差异改变了因蛋白冠状病毒而导致的体外巨细胞相互作用
Sridevi B Conjeevaram1, Amulya Kadaba1, Isaac M Adjei1
1Department of Biomedical Engineering, Texas A&M University, College Station, Texas 77843, United States.
ACS omega
|September 29, 2025
概括
纳米粒子表面化学的微妙变化显著改变蛋白质冠状形成和巨细胞相互作用. 这影响了纳米粒子生物分布和炎症反应,这对于临床应用至关重要.
科学领域:
- 生物医学工程 生物医学工程
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
背景情况:
- 纳米粒子 (NP) 与巨细胞的相互作用是它们体内命运的关键.
- 蛋白冠状 (PC) 形成影响NP-巨细胞相互作用,但表面化学效应通常被电荷和疏水性所混.
- 异构氨基酸提供了一种方法来隔离表面化学效应.
研究的目的:
- 研究纳米粒子表面化学对蛋白质冠状形成和巨细胞相互作用的影响.
- 为了区分表面化学的影响与电荷和疏水性.
- 了解如何微妙的表面修改影响NP命运 in vivo.
主要方法:
- 合成的金纳米粒子 (Au NPs) 经过氨酸 (Leu) 和异氨酸 (Iso Leu) 修改.
- 对于大小,泽塔潜力,疏水性和形态学的NP的特征.
- 在流动条件下形成蛋白质冠状体 (PC),并分析其组成.
- 进行了in silico路径分析和体外巨细胞吸收和细胞因子测试.
主要成果:
- 和Iso Leu修饰的Au NPs表现出类似的物理特性.
- 在 Leu-AuNP-PC 和 Iso Leu-AuNP-PC 之间,冠状蛋白的组成和数量各不相同.
- 在基分析中发现了明显的细胞和炎症通路激活.
- 巨细胞显示 Leu-AuNP-PC 的吸收较高,炎症性细胞因子的产生增加.
结论:
- 纳米粒子表面化学深度影响蛋白质冠状形成和随后的巨细胞相互作用.
- 微妙的化学差异可以导致NP吸收和炎症反应的显著变化.
- 这些发现对设计纳米粒子用于安全有效的临床应用具有重要意义.
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