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纳米孔封闭对FG重复的形态,动态和自组合特性的影响

Wancheng Zhao¹, Wai-Ming Yau¹, Robert Tycko¹

¹Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520.

bioRxiv : the preprint server for biology

|September 29, 2025

概括

核孔复合体 (NPC) 通道限制FG重复蛋白质,这对细胞功能至关重要. 这项研究表明,在纳米孔内连接这些FG重复序列可以防止聚合,保持对NPC传输至关重要的无序状态.

科学领域:

生物物理学的生物物理.
细胞生物学细胞生物学
材料科学材料科学材料科学

背景情况:

核孔复合体 (NPC) 调节了真核细胞中的运输.
FG重复蛋白域沿线NPC通道,调解分子流量.
在封闭的环境中了解FG重复行为是NPC功能的关键.

研究的目的:

开发一种方法来研究纳米孔内FG重复序列的研究.
研究FG重复结在纳米孔中的构造和自我组装特性.

主要方法:

使用具有20纳米孔隙的阳极氧化 (AAO) 晶片作为纳米孔隙支架.
通过酸盐-表面键,将FG重复 (FG30) 连接到孔壁.
使用定量13C和31P核磁共振 (NMR) 光谱和旋转放松测量.

主要成果:

在纳米孔中达到高度 (90 mM).
证明绑定的FG30链仍然具有动态无序和随机卷轴状.
观察到,在较低度 (2 mM) 的自由溶液中,FG30聚合物形成有序纤维.

结论:

阳极氧化 (AAO) 作为研究纳米孔环境中的多的有效支架.
将FG重复序列连接到纳米孔壁上,显著改变了它们的自组装行为.
这种方法为NPC中FG重复蛋白功能的受限作用提供了洞察力.

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