阿尔及利亚的比较分子分析和生物活性分析:抗氧化剂,抗菌活性,以及在中NRF2-KEAP1路径调节
Amel Reguig1, Ahmed Messai2, Ibtissam Kahina Bedaida3
1DEDSPAZA Laboratory, Department of Agronomical Sciences, Faculty of Exact Sciences and Natural and Life Sciences, University of Biskra, Biskra 07000, Algeria.
Current issues in molecular biology
|September 29, 2025
概括
阿尔及利亚在生物活性化合物,抗氧化能力和抗菌活性方面表现出显著的区域差异. 提取物2显示出卓越的抗氧化和抗微生物特性,具有药物发现的潜力.
科学领域:
- 自然产品 化学 化学
- 药理学 药理学是指药理学的学科.
- 微生物学 微生物学
背景情况:
- 蜂是一种天然蜜蜂产品,富含具有治疗潜力的植物化学物质.
- 阿尔及利亚仍然在很大程度上没有关于其化学成分和生物活性的特征.
- 区域气候差异可以显著影响的特性.
研究的目的:
- 研究来自不同生物气候地区的阿尔及利亚的分子特征,抗氧化能力和抗菌活性.
- 确定关键的生物活性化合物,并评估它们在药物发现方面的潜力.
- 为了比较来自潮湿的亚热带 (巴特纳) 和炎热的沙漠 (比斯克拉) 环境的.
主要方法:
- 电子喷射电离质谱仪 (ESI-MS) 是一种电子喷射电离质谱仪.
- 具有二极管阵列检测 (UHPLC-DAD) 的超高性能液态染色学
- 气色谱 - 质谱学 (GC-MS) 气色谱
- 抗氧化剂测定 (DPPH,ABTS,FRAP) 进行.
- 抗菌检测 (MIC,MBC) 是一种方法.
- 分子对接和in silico药物动力学预测
主要成果:
- 发现的成分存在显著的区域差异.
- прополис提取物2 (PE2) 的生物活性含量更高,抗氧化能力更强 (DPPH IC50: 27.74 μg/mL; FRAP: 5.16 μg/mL).
- PE2 显示出强大的杀菌作用,可以对抗金黄色葡萄球菌和大肠杆菌.
- 一种新型化合物,1,3-O-caffeoyl-dihydrocaffeoylglycerol,被确定具有强烈的KEAP1结合亲和力 (-11.02 Kcal/mol).
结论:
- 阿尔及利亚具有显著的抗氧化和抗菌潜力.
- 对于药物发现和开发来说,PE2是天然化合物的有希望的来源.
- 这项研究为进一步研究阿尔及利亚的应用提供了基础.
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